军事医学2017，Vol.41Issue(12)：968-972,977,6.DOI:10.7644/j.issn.1674-9960.2017.12.005
低剂量白介素2对实验性自身免疫性脑脊髓炎小鼠的治疗效果研究
Therapeutic effect of low dose interleukin-2 on experimental autoimmune encephalo-myelitis in mice
摘要
Abstract
Objective To investigate the therapeutic effect of interleukin-2(IL-2)on experimental autoimmune encephalomyelitis(EAE)mice.Methods After establishment of the EAE(experimental autoimmune encephalomyelitis) mouse models with MOG35-55 polypeptides,the mice were grouped according to the neurological function score and divided into control group,EAE group and low dose IL-2 treatment group.A double blind method was used to evaluate the neuro-logical impairment in mice.On the 29th day,pathological experiments were carried out in the mice's brain and spinal cord, hematoxylin-eosin staining was used to evaluate the scoring of inflammatory cell infiltration and luxol fast blue staining was used to evaluate the scoring of demyelinating.The proportion of regulatory T cells(Treg)and NK cells(natural killer cell, NK)was detected by flow cytometry,and the immunohistochemical method was used to detect the expressions of glial fibril -lary acidic protein(GFAP)and myelin basic protein(MBP)in the spinal cord.Results Compared with the EAE group, the neurological function score, the inflammatory cell infiltration score and the demyelinating score of the low dose IL-2 treatment group were reduced.The proportion of Treg cells in the low dose IL-2 treatment group was significantly higher than that in the EAE group,and the proportion of NK cells in the low dose IL-2 treatment group was slightly higher than that in the EAE group The expression of GFAP and MBP was detected by immunohistochemistry.The expression level of GFAP in low dose IL-2 treatment group was significantly lower than that in the EAE group,while the expression level of MBP was higher than that in the EAE group.Conclusion Low dose IL-2 has significant therapeutic effect on EAE mice.关键词
多发性硬化/脑脊髓炎,自身免疫性,实验性/调节性T细胞/白细胞介素2/神经胶质原纤维酸性蛋白/髓磷脂碱性蛋白质类Key words
multiple sclerosis/encephalomyelitis/autoimmune/experimental/regulatory T cell/interleukin-2/glial fibrillary acidic protein/myelin basic proteins分类
医药卫生引用本文复制引用
王震,段海峰,樊心童,许春阳,李金凤,王姗姗,王运良,吴祖泽..低剂量白介素2对实验性自身免疫性脑脊髓炎小鼠的治疗效果研究[J].军事医学,2017,41(12):968-972,977,6.基金项目
河南省科技发展计划资助项目(162102310557) （162102310557）
