军事医学2017，Vol.41Issue(12)：983-986,4.DOI:10.7644/j.issn.1674-9960.2017.12.008
黄芪注射液对甲状腺辐射损伤改善作用的实验研究
Effect of Astragalus injection on thyroid radiation injury
王津京 1侯艳微 1张世坤 2刘然 3曾静 1李致远 1方毅1
作者信息
- 1. 解放军307医院,北京 100071
- 2. 安徽省立医院病理科,合肥 230001
- 3. 军事科学院军事医学研究院军事认知与脑科学研究所,北京 100850
- 折叠
摘要
Abstract
Objective To investigate the effect of Astragalus injection on iodine-131(131 I)induced thyroid radiation injury.Methods Two-stage SD male rats were randomly divided into three groups: control group, 131I irradiation group and Astragalus intervention 131I irradiation group.131I irradiation group and Astragalus intervention 131I irradiation group were treated with intragastric administration of 11.1MBq 131I, respectively.At the same time, the Astragalus intervention 131 I irradiation group was injected intraperitoneally 400 mg/(kg· d)Astragalus injection liquid.The levels of thyroid hormone were measured by solid-phase radioimmunoassay in the 2nd and 8th weeks of the experiment.The thyroid tissues from rats were HE stained into paraffin sections after 8 weeks.Administration of 0,25,50,100,200 MBq/ml into 131I irradiation of thyroid follicular carcinoma cells(WRO)lasted 24 hours, the proliferation and apoptosis of WRO in Astragalus membranaceus 0.5 g/L intervention and non-Astragalus intervention were detected by MTT assay and flow cytometry.Results Compared with the normal control group, FT3and FT4were significantly decreased in the 131 I irradiation group(P=0.021,0.017).The morphological changes of the follicular epithelial cells in the thyroid tissue were irregular and the hyaline degeneration was observed.However, compared with 131I irradiation group, FT3and FT4were significantly improved by Astragalus injection(P=0.033,0.045),and the degree of vitreous degeneration of thyroid tissue was alleviated.Cell experiments in vitro showed that the proliferation of thyroid cells was increased, but apoptosis was reduced.Conclusion Astragalus injection can improve the thyroid function and thyroid injury induced by 131 I in rats.关键词
甲状腺/辐射损伤/黄芪/碘/甲状腺激素类/甲状腺功能减退症Key words
thyroid gland/radiation injuries/Astragalus membranaceus/iodine/thyroid hormones/hypothyroidism分类
医药卫生引用本文复制引用
王津京,侯艳微,张世坤,刘然,曾静,李致远,方毅..黄芪注射液对甲状腺辐射损伤改善作用的实验研究[J].军事医学,2017,41(12):983-986,4.
