肿瘤预防与治疗2017，Vol.30Issue(6)：427-432,6.DOI:10.3969/j.issn.1674-0904.2017.06.006
p16INK4a/Ki67免疫细胞化学双染在检出宫颈癌及癌前病变中的作用
Detection of Cervical Cancer or Precancerous Lesion by p16INK4a/Ki67 Dual Stain Cytology
摘要
Abstract
Objective:To investigate the effect of p16INK4a/Ki67 dual stain cytology on the detection of cervical cancer or precancerous lesion.Methods:The cervical cytology,hr HPV test,p16INK4a/Ki67 dual stain cytology and biopsy were performed for a total of 131 women with an average age of 39 ± 10.73 in outpatient of gynecologic department from July 2016 to Jan.2017.CINtec Plus kit was used for p16INK4a/Ki67 dual stain cytology.Thinprep or Surepath liquid based cytology was applied for cytology test.Cobas 4800 system was chosen for hrHPV test.Results:The sensitivity and specificity of p161NK4a/Ki67 dual stain cytology (92.8％,58.8％) were significantly higher than those of the liquid based cytology (82.5％,44.1％),and its specificity was much higher than that of rhHPV test (17.6％,P ＜ 0.05) when CIN2 was set as the cutoff.p16INK4a/Ki67 dual stain cytology has the highest AUC (0.773) among these three methods (P ＜ 0.05).Combination of p16INK4a/Ki67 dual stain cytology and cervical liquid based cytology got the lowest omission diagnostic rate for CIN2 and greater lesions.Conclusion:p16INK4a/Ki67 dual stain cytology has relatively higher sensitivity and specificity,thus can help decrease the omission diagnostic rate and over-diagnosis rate of high grade cervical squamous epithelial lesions,as well as improve the detection rate of cervical cancer.关键词
p16INK4a/Ki67双染/宫颈细胞学/高危型HPV检测/CIN/宫颈癌Key words
p16INK4a/Ki67 dual stain/Cervical cytology/hrHPV test/CIN/Cervical cancer分类
医药卫生引用本文复制引用
张睿怡,郭红燕,游珂,郭艳利,李子健,耿力..p16INK4a/Ki67免疫细胞化学双染在检出宫颈癌及癌前病变中的作用[J].肿瘤预防与治疗,2017,30(6):427-432,6.基金项目
国家自然科学基金(编号:81472429) （编号:81472429）
