山东农业科学2017，Vol.49Issue(12)：110-113,4.DOI:10.14083/j.issn.1001-4942.2017.12.026
凝乳酶热失活动力学研究
Study on Thermal Deactivation Kinetics of Rennin
摘要
Abstract
To study the thermal deactivation kinetics behavior of rennin,the Arrhenius equation was used to obtain the deactivating rate constant (k) and half-life (t1/2) at different temperatures (334 ～ 343 K).Then,the activation energy (Ea) of deactivated rennin was obtained,which was (539.58 ± 1.55) kJ/mol.When the temperature was in the range of 335 ～ 343 K,the deactivating rate constants could be well fitted with linear regression,which suggested that the deactivation of rennin was in accordance with the first-order kinetics model.At the lower temperature of 334 K,the curve of deactivating rate constant had lower linear fitting degree.The deactivating rate constant was on the high side at earlier stage but on the low side at later stage of heat preservation,and the curve slope was significantly different between earlier and later stages,which indicated that transition-state intermediate might be existent in the deactivation process of rennin.关键词
凝乳酶/热失活动力学/热稳定性Key words
Rennin/Thermal deactivation kinetics/Thermostability分类
农业科技引用本文复制引用
武彬,刘雪兰,石天虹,阎佩佩,伦忠杰,王昭霖,井庆川..凝乳酶热失活动力学研究[J].山东农业科学,2017,49(12):110-113,4.基金项目
山东省优秀青年科学家奖励基金项目(BS2011SW041) （BS2011SW041）
山东省现代农业产业技术体系家禽创新团队建设项目(SDAIT-11-06) （SDAIT-11-06）
