山东农业科学2017，Vol.49Issue(12)：126-129,4.DOI:10.14083/j.issn.1001-4942.2017.12.029
三种血清学布鲁氏菌检测方法在不同疫苗免疫奶牛中的比较
Comparative Study of Antibodies from Vaccinated Cows with Different Vaccines against Brucellosis Using Three Kinds of Serological Detection Methods
摘要
Abstract
Brucellosis is a serious zoonosis that occurs in worldwide.Vaccine and sero-detection are two effective and common methods for its control in dairy herds.However,its sero-diagnosis is interfered by vaccine immunization,so it is important to know the dynamic changes of antibodies after being immunized with different vaccines based on various detection methods.Fifteen six-month-old healthy cows were randomly divided into five groups on average.Four treatment groups were inoculated by injection and oral immunization of 6 × 1010 unit A19 vaccine and 5 × 1010 unit S2 vaccine,respectively.Every group was separately kept after vaccination and environmental disinfection.The serum samples were monthly collected in six months and detected by RBPT,iELISA and cELISA.The results showed that the injected groups had a higher antibody peak level and longer antibody duration time compared with oral groups,and the S2 groups had a higher antibody peak level and shorter antibody duration time than A19 groups.No antibody was detected for A19 oral group by three methods in six months.All the results showed that vaccine immunization had obvious interference effect on sero-diagnosis.But the influencing time on cELISA was short,thus the vaccine immunization and wild strain infection could be effectively distinguished by cELISA.For different vaccines with different modes of immunization,the appropriate time node should be selected.关键词
布鲁氏菌病/疫苗/抗体消长规律/检测方法Key words
Brucellosis/Vaccines/Dynamical changes of antibody/Detection method分类
农业科技引用本文复制引用
张亮,王基隆,丁家波,解晓莉,刘来兴,谷祖烨,杨美,杨宏军..三种血清学布鲁氏菌检测方法在不同疫苗免疫奶牛中的比较[J].山东农业科学,2017,49(12):126-129,4.基金项目
山东省自然科学基金面上项目“布鲁氏菌T4SS效应蛋白BPE123和BPE275的生物功能研究”(ZR2014CM008) （ZR2014CM008）
山东省自然科学基金三院联合项目“牛型分枝杆菌ESAT6和CFP10调控巨噬细胞炎症反应的功能研究”(ZR2015YL069) （ZR2015YL069）
“十三五”国家重点研发计划子课题“布鲁氏菌病和牛结核病的诊断与检测新技术研究”(2016YFD0500902) （2016YFD0500902）
现代农业(奶牛)产业技术体系科学家岗位项目(CARS-36) （奶牛）
