山东医药2018，Vol.58Issue(6)：11-14,4.DOI:10.3969/j.issn.1002-266X.2018.06.003
阿胶对气道炎症小鼠Th17/Treg亚群失衡的逆转作用
Reverse effect of Ejiao (colla corii asini) on imbalance of Th17/Treg subsets of mice with airway inflammation
摘要
Abstract
Objective To investigate the therapeutic effect of Ejiao (colla corii asini) on the imbalance of Th1T/Treg subsets in the lung tissues of mice with smoking-induced airway inflammation.Methods Twenty-four male C57BL/6 mice were randomly divided into the control group,model group,and Ejiao (colla corii asini) group (Ejiao group).Mice in the model group and Ejiao group were exposed to cigarette smoking.Mice in the model groupwere administered 0.2 mL PBS daily,mice in the Ejiao group were administered 0.2 mL Ejiao (colla corii asini) solution (0.2g/ml) daily,and mice in the control group were fed normally.Twenty-four weeks later,the histopathological condition of lung in mice of each group was observed.The proportion of helper T cell 17 subsets (Th17) and regulatory T cell subsets (Treg) in the lung tissues were detected by flow cytometry.The expression of IL-6,IL-17A,and Foxp3 mRNA in the lung tissues was detected by reverse transcription polymerase chain reaction (RT-PCR),and the serum levels of IL-6,IL-17A,and Foxp3 were detected by enzyme-linked immunosorbent assay.Results Compared with the control group,the lung tissues of mice in the model group were infiltrated with large number of inflammatory cells,alveoli ruptured and fused,the proportion of Th17 cells and Treg cells in the lung tissue increased,and the levels of IL-17A,IL-6,Foxp3 in the serum,and IL-17A,Foxp3,and IL-6 mRNA in the lung tissues increased (all P ＜ 0.05).Compared with the model group,the inflammation of the lung tissues of mice in the Ejiao group was significantly improved,the proportion of Th17 cells and Treg cells in the lung tissues decreased,and the levels of serum IL-17A,IL-6,and Foxp3,and IL-17A,Foxp3,and IL-mRNA in the lung tissues decreased (all P ＜ 0.05).Conclusions Smoke can induce the increased proportion of Th17 and Treg subsets and up-regulate the expression of IL-6,IL-17A,and Foxp3 cytokines in the lung tissues of mice with airway inflammation.Ejiao (colla corii asini) can reduce the lung inflammation in mice by reducing the proportion of Thl7 and Treg subsets and expression of IL-6 and IL-17A,and Foxp3.关键词
香烟暴露/阿胶/气道炎症/辅助型T细胞17型/调节性T细胞Key words
cigarette smoking exposure/Ejiao (colla corii asini)/airway inflammation/helper T cell 17 subsets/regulatory T cells分类
医药卫生引用本文复制引用
张喆,马云,胡晶红,兰红云,张永清,姚成芳..阿胶对气道炎症小鼠Th17/Treg亚群失衡的逆转作用[J].山东医药,2018,58(6):11-14,4.基金项目
国家自然科学基金资助项目(81573728) （81573728）
山东省自然科学基金资助项目(ZR2015HM007) （ZR2015HM007）
山东省医药卫生科技发展计划项目(2015WS0192) （2015WS0192）
山东省重点研发计划项目(2015GSF119004). （2015GSF119004）
