世界中医药2017，Vol.12Issue(11)：2731-2735,5.DOI:10.3969/j.issn.1673-7202.2017.11.042
益气解毒方基于线粒体保护的抗缺血性脑卒中研究
Study on Effects of Yiqi Jiedu Fang on Mitochondrial Metabolism-related Energy Function in Ischemic Stroke Rats
摘要
Abstract
Objective:To study the effects of Yiqi Jiedu Fang on mitochondrial energy metabolism related functions in ischemic stroke rats,and to explore the possible protective mechanisms of Yiqi Jiedu Fang on ischemic stroke through intervening the mitochondrial energy metabolism disorder.Methods:Middle Cerebral Artery Occlusion (MCAO) was used.Yiqi Jiedu Fang and its separated formulas were given 15 min prior to MCAO.After 12 h of modeling,mitochondria were extracted.Indexes of mitochondrial three-state respiration rate,four-state respiratory rate,phosphorus-oxygen ratio,respiratory control rate,oxidative phosphorylation rate and other indicators were determined.The protective effect of Yiqi Jiedu Fang on ischemic stroke was evaluated.In addition,the effect of each component of Yiqi Jiedu Fang on anti-ischemic stroke was evaluated by Principal Component Analysis (PCA) and Variable Importance in Projection (VIP).Results:Yiqi Jiedu Fang can improve the mitochondrial respiration related indexes during the pathological process of ischemic stroke.The separated formula of Yiqi Jiedu Fang also has a better effect on ischemic stroke.PCA results showed that compared with other separated formula groups,Yiqi Jiedu Fang group had the best treatment effect.In each separated formula groups,the compatibility of Radix et Rhizoma Ginseng,Fructus Gleditsiae and berberine had the best treatment effect.The results of VIP analysis showed that berberine had the largest contribution to the pathological process of ischemic stroke based on the protection of mitochondrial respiratory function.Conclusion:Yiqi Jiedu Fang can significantly reduce the pathological damage caused by ischemic stroke,whose mechanism may be related to the protection of mitochondrial function.关键词
益气解毒方/脑卒中/线粒体Key words
Yiqi Jiedu Fang/ Stroke/ Mitochondrial分类
医药卫生引用本文复制引用
杨若聪,田朋朋,吴传鸿,段飞鹏,陈修平,张丽红,闫智勇,李韶菁..益气解毒方基于线粒体保护的抗缺血性脑卒中研究[J].世界中医药,2017,12(11):2731-2735,5.基金项目
国家自然科学基金项目(81274133,81303261) （81274133,81303261）
国家科技重大专项(重大新药创制)资助项目(2012ZX09103201-055) （重大新药创制）
