世界中医药2017，Vol.12Issue(11)：2771-2774,4.DOI:10.3969/j.issn.1673-7202.2017.11.052
白花蛇舌草对大肠癌耐药细胞microRNAs表达的影响
Effects of Herba Hedyotis Diffusae on Expression of microRNAs in Colorectal Cancer Drug Resistant Cells
摘要
Abstract
Objective:To study the regulatory effect of Herba Hedyotis Diffusae on multidrug-resistance (MDR)-related microRNAs (miRNAs) in colorectal cancer,and further explore the underlying mechanisms of its MDR reversion.Methods:The signal pathway regulated by miRNA was forecasted by MTT assays,morphology observation,miRNA array analysis,QPCR validation,GO and Pathway analysis through treatment of HDW in HCT-8/5-FU cells.Results:HDW significantly inhibited colorectal cell viability and decreased density of HCT-8/5-FU cells.There were 23 up-regulated and 34 down-regulated in 57 miRNA differential expression after HDW treatment;and QPCR verification confirmed that HDW increased expression of miR-92b-5p,miR-1247-3p and miR-4800-5p and decreased expression of miR-4454,miR-4443,miR-483-5p;and the signal pathway prediction showed that HDW could control PI3 K/Akt,TGF-β/Smad,MAPK,P53,Wnt and JAK-STAT signaling pathways regulating MDR,migration,invasion,proliferation and apoptosis.Conclusion:HDW can remarkably suppress proliferation in HCT-8/5-FU cells,and it is one of the mechanisms to reverse the drug resistance of colorectal cancer by regulating miRNAs expression.关键词
白花蛇舌草/大肠癌/microRNA/耐药Key words
Herba Hedyotis Diffusae/Colorectal cancer/microRNA/Drug resistance分类
医药卫生引用本文复制引用
林久茂,李琼瑜,严兆坤,赖子君,靳祎祎,彭军..白花蛇舌草对大肠癌耐药细胞microRNAs表达的影响[J].世界中医药,2017,12(11):2771-2774,4.基金项目
教育部博士点基金博导类项目(20133519110003) （20133519110003）
陈可冀中西医结合发展基金项目(CKJ2015007,CKJ2014013) （CKJ2015007,CKJ2014013）
