世界中医药2017，Vol.12Issue(11)：2775-2778,4.DOI:10.3969/j.issn.1673-7202.2017.11.053
黄连多糖中单糖组成的HPLC-MSn法快速识别
Monosaccharide Composition Analysis of Polysaccharides from Rhizoma Coptidis Based on HPLC-MSn
摘要
Abstract
Objective:To explore a rapid identification of monosaccharide composition of polysaccharide from Rhizoma Coptidis through HPLC-MSn.Methods:After being extracted,separated,removed of protein and pigment,Rhizoma Coptidis polysaccharide was obtained from Rhizoma Coptidis.Polysaccharides of Rhizoma Coptidis was hydrolyzed with the TFA of 2 mol.L-1.1-phenyl-3-methyl-5-pyrazolone (PMP) was used as derivatization reagent.And high performance liquid chromatography-mass spectrometry (HPLC-MSn) was used to identify the composition of monosaccharide,and the resolve fragmentation regularity of its derivatives was analyzed.Results:Through analysis,the monosaccharides of polysaccharides from Rhizoma Coptidis were mannose,ribose,rhamnose,galacturonic acid,glucose,galactose,arabinose/xylose,fucose,among which ribose and fucose were the first reported monosaccharides that obtained from polysaccharide of Rhizoma Coptidis.Conclusion:HPLC-MSn has a guiding significance in analyzing the monosaccharide composition of polysaccharides from Chinese materia medica.关键词
黄连多糖/PMP/单糖/HPLC-MSnKey words
Rhizoma Coptidis polysaccharide/PMP/Monosaccharide/HPLC-MSn分类
医药卫生引用本文复制引用
张亚丽,高简,苗祥贞,张潇,刘永刚,谭鹏..黄连多糖中单糖组成的HPLC-MSn法快速识别[J].世界中医药,2017,12(11):2775-2778,4.基金项目
青年科学基金项目(81102807) （81102807）
