生命科学研究2018，Vol.22Issue(1)：36-41,6.DOI:10.16605/j.cnki.1007-7847.2018.01.006
人源LDHA基因启动子质粒的构建及在肺癌细胞中Nrf2对其转录表达调控和功能分析
Construction of Human LDHA Gene Promoter Plasmid and Analysis of Its Transcriptional Regulation and Function by Nrf2 in Lung Cancer Cells
摘要
Abstract
In order to investigate the effect of nuclear factor erythroid 2-related factor 2 (Nrf2) on tumor metabolism and,consequently,tumor migration,we focused on regulation of lactate dehydrogenase A (LDHA) expression by Nrf2.Herein,the plasmid LDHA-luc containing the firefly luciferase reporter gene driven by human LDHA gene promoter was successfully constructed,and transfected into A549 cells together with Nrf2 expresion vector.After transfection,the dual luciferase reporter gene assay system showed that the LDHA promoter activity in cells transfected with Nrf2 plasmid was significantly higher than that of the control.Meanwhile,the expression level of LDHA in cells transfected with Nrf2 plasmid was also higher,indicating that Nrf2 promoted transcription and expression of LDHA in A549 cells.Up-regulation of LDHA promotes the formation of acidic tumor microenvironment and cell migration.Therefore,successful construction of the plasmid LDHA-luc and demonstration of LDHA gene expression regulation by Nrf2 lay a foundation for further research on how Nrf2 influences tumor metabolism to promote cell migration through LDHA.关键词
乳酸脱氢酶A (LDHA)/核因子E2相关因子2(Nrf2)/萤火虫荧光素酶报告基因质粒/转录表达分析/细胞迁移Key words
lactate dehydrogenase A (LDHA)/nuclear factor erythroid 2-related factor 2 (Nrf2)/firefly luciferase reporter gene plasmid/transcriptional expression analysis/cell migration分类
生物科学引用本文复制引用
曹玉祥,周兴路,李祥瑞,黄滔,柯丽霞,吴志浩..人源LDHA基因启动子质粒的构建及在肺癌细胞中Nrf2对其转录表达调控和功能分析[J].生命科学研究,2018,22(1):36-41,6.基金项目
国家自然科学基金面上项目(81272359) （81272359）
皖南医学院高层次引进人才启动基金 （）
