实用医学杂志2018，Vol.34Issue(1)：16-20,25,6.DOI:10.3969/j.issn.1006-5725.2018.01.005
二氯乙酸对膀胱癌细胞株T24克隆形成、侵袭和迁移的影响
Effect of dichloroacetate on colony-forming, invasion and migration of the T24 bladder cancer cells line and its mechanism
摘要
Abstract
Objective To study the effects of dichloroacetate (DCA) on cell colony-forming,cell invasion and cell migration of the bladder cancer cells and to study the underlying mechanism.Methods The bldder cancer cells T24 were randomly divided into two groups:the observation group and the control group.Cells in the observation groups were treated with 5 mmol/L,10 mmol/L and 20 mmol/L dichloroacetate,and the control group was treated with the same amount of dimethyl sulfoxide.Colony formation assays were detected with Giemsa staining.Cell wound scratch assay and Transwell assay were applied to evaluate the ability of the T24 cell invasion and migration.Realtime PCR and Western blot were applied to detect the expression of the epithelial-mesenchymal transition (EMT)-related marker,including E-cadherin,N-cadherin,vimentin,Snail and Slug.Results Compared with the control group,the colony formation assays of T24 cells constantly decreased along with the increased doses in the observation group(P ＜ 0.01).The cell wound scratch assay showed that the scratch width of the observation groups were significantly higher along with the increased doses and prolonged time than that in the control group (P ＜ 0.01).The transwell assay showed that the invasion ability of the observation groups were significantly discreased along with the increased doses than that in the the control group (P ＜ 0.01).The expression levels of E-cadherin mRNA and protein in combination the control group were higher than those in the the observation groups (P ＜ 0.05).However,the expression levels of N-cadherin,vimentin,Snail and Slug mRNAs and proteins in combination the control group were lower than those in the the observation groups (P ＜ 0.05).Conclusion Dichloroacetate can inhibit the colony-forming,invasion and migration of bladder cancer T24 cells,and its mechanism may inhibit the expression of epithelial mesenchymal transition in T24 cells by down-regulating the expression of nuclear transcription factor Snail and Slug.关键词
二氯乙酸/膀胱癌/克隆形成/侵袭/迁移/上皮间质转化Key words
dichloroacetate/bladder cancer/colony-forming/invasion/migration/epithelial-mesenchymal transition引用本文复制引用
谢智彬,付伟金,陆春宇,赵东,徐艳圳,吴华裕..二氯乙酸对膀胱癌细胞株T24克隆形成、侵袭和迁移的影响[J].实用医学杂志,2018,34(1):16-20,25,6.基金项目
广西自然科学基金(编号:2015GXNSFAA139180) （编号:2015GXNSFAA139180）
广西高校中青年教师基础能力提升基金(编号:2017KY0111) （编号:2017KY0111）
