同济大学学报（自然科学版）2018，Vol.46Issue(2)：241-246,6.DOI:10.11908/j.issn.0253-374x.2018.02.015
基于细胞培养的新型微流体加热器的开发与应用
Exploitation and Application of a Novel Integrated Microfluidics Heater for Cell Cutlure
摘要
Abstract
A functional and robust microfluidic perfusion-based 2-D cell culture system was developed for mammalian cells (Hela) with long term monitoring,which provided a closed environment for cell growth and manipulation without the incubator.A 9-chamber poly (dimethylsiloxane) (PDMS) flow system with multi-layer quake-valve control was integrated with a microheater on an Aurum/Chromium (Au/ Cr) wafer.Based on a valve control and bypass,each chamber worked independently by feeding cells with the desired density into the chamber or chasing cells out of it.With the novel network microheater,a steady temperature of 37 ℃ was guaranteed for cells.Results show that cells grow as well as those based on the conventional tissue cultural vessels.关键词
微流体系统/微加热系统/装置设计/细胞培养Key words
microfluidics/microheater/device designing/mammalian cells分类
能源科技引用本文复制引用
张艳秋,陶文铨,李卓..基于细胞培养的新型微流体加热器的开发与应用[J].同济大学学报（自然科学版）,2018,46(2):241-246,6.基金项目
国家自然科学基金(51676141,21307092) （51676141,21307092）
中央高校基本科研业务费专项资金(0400219381) （0400219381）
