西北国防医学杂志2018，Vol.39Issue(2)：71-74,4.DOI:10.16021/j.cnki.1007-8622.2018.02.001
快速复制大鼠非酒精性脂肪性肝病模型及主要脏器的病理变化
Pathological changes of the organs in rats in the process of building the non-alcoholic fatty liver disease model
摘要
Abstract
Objective:To explore the method of establishing nonalcoholic fatty liver model in rats in a relatively short period of time,and to observe the pathological changes of the organs in rats.Methods:Twenty-four SD male rats were randomly divided into 3 groups,including the control group(intragastric infusion of saline,10 ml/kg and interperitoneal injection of paraffin oil,3 ml/kg,fed with common diet),CCL4 group(intragastric infusion of saline,10 ml/kg and interperitoneal injection of 40％ CCL4 paraffin oil,3 ml/kg,fed with common diet),and experimental group(intragastric infusion of high fat diet and high cholesterol food,10 ml/kg and interperitoneal injection of by 40％,3 ml/kg,fed with common diet).These treatments were both twice a week.On the third weekend,all rats were killed to observe the pathological changes of the organs including the liver,cardiac,kidney and spleen by HE dyeing.Results:High fat and high cholesterol food can cause obvious fatty degeneration in liver cells within 21 days,but it has little effect on other organs.Conclusion:The method that high fat and cholesterol food lavage can build the nonalcoholic fatty liver disease model in rats in a relatively short of time,greatly shortens the experimental period,has the grate value in the basic experiment.关键词
非酒精性脂肪性肝病/病理变化/模型/大鼠Key words
nonalcoholic fatty liver disease/pathological changes/model/rats分类
医药卫生引用本文复制引用
黄婷婷,赵丽娟,姜双燕,李敏,杨洋,陈莎莎..快速复制大鼠非酒精性脂肪性肝病模型及主要脏器的病理变化[J].西北国防医学杂志,2018,39(2):71-74,4.基金项目
辽宁省自然科学基金资助项目(201602336) （201602336）
