西北国防医学杂志2018，Vol.39Issue(2)：75-78,4.DOI:10.16021/j.cnki.1007-8622.2018.02.002
肢体缺血处理对缺血再灌注大鼠心肌PPARα mRNA和蛋白表达的影响
Impact of repetitive limb-ischemia conditioning on expressions of PPARα mRNA and protein in myocardium of rats following myocardial ischemia-reperfusion injury
摘要
Abstract
Objective:To observe the levels of CK-MB、cTnI and the expressions of peroxisome proliferator activated receptor-α(PPARα) mRNA and protein in myocardium of rats following myocardial ischemia-reperfusion(IR) injury and the impact of limb-ischemia (LI) conditioning for three times during different myocardial ischemic period.Methods:Fourty Wistar rats were randomly divided into 4 groups:IR group,LI-perC group (LI perconditioning),LI-preC group (LI precondioning),LI-postC group (LI postcondioning),ten rats in each group.Serum specimens and injured cardium of rats in four groups were kept after treatment.The contents of CK-MB and cTnI in the serum were detected according corresponding kits.The expressions of PPARα mRNA and protein in the myocardium were evaluated by reverse transcription polymerase chain reaction (RT-PCR) and Western blot respectively.Results:The levels of CK-MB and cTnI in LI-perC group,LI-preC group and LI-postC were significantly lower than those in IR group(P＜0.05),while the expressions of PPARα mRNA and protein in the three groups were significantly higher than those in IR group (P＜0.05).There were no significant changes in the contents of CK-MB and cTnI,the expressions of PPARα mRNA and protein in LI-perC group,LI-preC group and LI-postC group (P ＞0.05).Conclusion:.Limb-ischemia conditioning during different myocardial ischemic period may decrease myocardial injury and increase the expressions of PPARα mRNA and protein.关键词
心肌缺血再灌注损伤/肢体缺血处理/过氧化物酶体增殖物激活因子αKey words
myocardial ischemia-reperfusion injury/limb-ischemia conditioning/peroxisome proliferator activated receptor-α分类
医药卫生引用本文复制引用
任冰稳,张宝和,翟鑫,胡智,朱哲宇..肢体缺血处理对缺血再灌注大鼠心肌PPARα mRNA和蛋白表达的影响[J].西北国防医学杂志,2018,39(2):75-78,4.基金项目
海军总医院创新培育基金资助项目(CXPY201534) （CXPY201534）
