西北林学院学报2018，Vol.33Issue(2)：20-27,8.DOI:10.3969/j.issn.1001-7461.2018.02.04
钙对马尾松针叶生理生化特性及细胞超微结构的影响
Effects of Calcium Concentration on Physio-biochemical Characteristics and Ultrastructure of Pinus massoniana Needles
摘要
Abstract
To provide reference for Pinus massoniana plantation management,a greenhouse sand culture experiment was designed to observe physico-biochemical responses and cell ultrastructure of P.massoniana needles in different Ca2+ concentrations (0,0.4,1,2,3 and 4 mmol · L-1) and to illuminate the adapting mechanisms of P.massoniana seedlings to different Ca2+ concentrations.The results indicated that the seedling's biomass,needle's chlorophyll,GA,ZR and IAA contents and ratio of chlorophyll a/b increased and then decreased with the increase of Ca2+ concentrations,and all their maximum values appeared in 2 mmol · L-1 Ca2+,but the rule was varied inversely with the needle's relative conductivity rate,ABA content and ratio of ABA/GA,ABA/ZR,ABA/IAA and ABA/(GA+ZR+IAA).For the mesophyll cell of P.massoniana with 2 mmol · L-1 Ca2+,chloroplasts and mitochondria were all complete,its content of starch was higher,osmiophilic granule and mitochondrion all had lower contents and all cells had no caused plasmolysis.Nevertheless,mesophyll cells of other 5 treatments all appeared plasmolysis at different calcium levels,and which finally caused cellular structure damage and function disorder.Mesophyll cells of P.massoniana under 0 mmol · L-1 Ca2+ was most severely damaged,and appeared cavitations within chloroplasts.The next was P.massoniana with 4 mmol · L-1 Ca2+ according to the degree of cellular structure damage.Thus,P.massoniana seedling treated with different Ca2+ levels could regulate endohormone contents and their comparative ratios,physiological metabolism and photosynthesis,to protect the normal structure and function of cell membrane and organelles,effectively to promote seedling growth.The treatment of 2 mmol · L-1 Ca2+ concentration was the best for P.massoniana seedlings.关键词
马尾松/钙浓度/生理生化特性/超微结构Key words
Pinus massoniana/calcium concentration/physio-biochemical index/ultrastructure分类
农业科技引用本文复制引用
李德燕,周运超..钙对马尾松针叶生理生化特性及细胞超微结构的影响[J].西北林学院学报,2018,33(2):20-27,8.基金项目
贵州省重大专项(黔科合重大专项字[2012]6001号) （黔科合重大专项字[2012]6001号）
“百”层次人才计划(黔科合人才(2015)4022). （黔科合人才(2015）
