陕西科技大学学报2018，Vol.36Issue(2)：74-79,6.
液晶传感竞争免疫法检测天蚕素B
Label-free liquid crystal immunosensor for detection of cecropin B
摘要
Abstract
A label-free liquid crystal immunosensor based on orientation changes of liquid crystals was prepared for the detection of cecropin B through competitive immunization as-say.The mixed APTES/DMOAP self-assembled monolayers formed by both long and short alkyls could cause uniform vertical alignment of liquid crystals.The homeotropic-to-tilted transition of liquid crystals induced by the specific binding event between anti-cecropin B an-tibody and cecropin Bimmobilized via a cross-linker glutaradehyde could result in the corre-sponding changes of the color and brightness of liquid crystal film.The gray-scale intensities of optical images of liquid crystal cells were measured to quantitatively analyse cecropin B concentrations at the range of 0.01~0.1 ng/mL and as a result,the limit of detection is as low as 0.01 ng/mL with immobilized cecropin B concentration of 150 ng/mL and anti-cecropin B antibody concentration of 500 ng/mL.This study offers a simple,highly sensitive and specific,lable-free method for cecropin B detection.关键词
液晶/自组装膜/免疫传感器/天蚕素B/天蚕素B抗体Key words
liquid crystal/self-assembled film/immunosensor/cecropin B/anti-cecropin B antibody分类
化学化工引用本文复制引用
苏秀霞,张姣,栾崇林,霍文静,徐佳..液晶传感竞争免疫法检测天蚕素B[J].陕西科技大学学报,2018,36(2):74-79,6.基金项目
国家自然科学基金项目(21175037) （21175037）
广东省自然科学基金项目(2016A030313847) （2016A030313847）
