畜牧兽医学报2018，Vol.49Issue(3)：507-514,8.DOI:10.11843/j.issn.0366-6964.2018.03.006
贵妃鸡多趾性状遗传规律分析及多趾候选基因的表达
Genetic Analysis of Polydactyly Traits in Royal Chicken and Its Candidate Genes Expression
摘要
Abstract
The purpose of this study was to explore the genetic rules of polydactyly traits and the expression of its candidate genes during embryogenesis in order to provide a basis for the selection and breeding of polydactyly traits lines in Royal chicken.Royal chicken with different toe types (four-four toe,four-five toe,five-five toe) were selected,and hybridization and crossbreeding test were carried out to analyze the proportions of various toe types in offsprings,then it was compared with the theoretical values by Chi square test.The limb bud tissue samples from 5th to 10th day during embryonic development were collected and the expression of the polydactyly traits candidate genes(Lmbr1,SHH,RNF32,NOM1,MNX1 and Gli3) were analyzed.The results showed that the ratio of polydactyly to normal dactyly in four-five toe ♂ × four-five toe ♀-group was 3.67 ∶ 1,which was in accordance with the theoretical ratio of 3 ∶ 1 (P＞0.05).The other two groups did not fit the theoretical ratio (P＜0.05).Secondly,the result of crossbreeding test showed that there were 3 toe types in offsprings from each cross male.Finally,the expression of SHH gene was very low from the 5th to 10th day during the development of limb bud tissue in Royal chicken.Lmbrl,RNF32,NOM1,MNX1,Gli3 gene expression peaks appeared on the 7th and 9th day during the development of limb bud tissue.The polydactyly trait of Royal chicken is in form of autosomal dominant inheritance,but its penetrance is different.The results indicate that the polydactyly traits may be regulated by multiple genes such as Lmbr1 and RNF32,and the critical period may be from the 7th to 9th day during embryo development.关键词
贵妃鸡/多趾性状/遗传规律/基因表达Key words
Royal chicken/polydactyly trait/genetic rule/gene expression分类
农业科技引用本文复制引用
王香南,郭红威,郭亚苹,闫峰宾,王彦彬,孙桂荣,康相涛,韩瑞丽..贵妃鸡多趾性状遗传规律分析及多趾候选基因的表达[J].畜牧兽医学报,2018,49(3):507-514,8.基金项目
国家蛋鸡产业技术体系遗传育种岗位专项资金(CARS-40-K04) （CARS-40-K04）
教育部创新团队发展计划(IRT16R23) （IRT16R23）
河南省产学研合作项目(162107000066) （162107000066）
