畜牧兽医学报2018，Vol.49Issue(3)：525-533,9.DOI:10.11843/j.issn.0366-6964.2018.03.008
绵羊FGF 7基因组织表达及其多态性与产羔数之间的关系
Tissue Expression and Polymorphism of Sheep FGF 7 Gene and Their Association with Litter Size
摘要
Abstract
To elucidate the association between tissue expression level and polymorphism of FGF 7 and litter size in sheep,semi-quantitative reverse transcription polymerase chain reaction (sqRT-PCR) and quantitative real-time PCR (qPCR) were performed to investigate the expression of FGF 7 gene in multiparous Small Tail Han sheep and uniparous Sunite sheep and different genotypes of FecB gene in Small Tail Han sheep.Multiparous (380 Small Tail Han sheep) and uniparous (total 380 for Tan,Sunite,Suffolk,Dorper and Prairie Tibetan sheep) sheep breeds were selected,and the locus of g.57842893C＞T in FGF 7 gene was genotyped using Sequenom MassARRAY~ SNP assay.Then the association was analyzed between FGF 7 gene and litter size in Small Tail Han sheep.The results showed that the expression of FGF 7 was at high level in heart and lung in both uniparous and multiparous sheep breeds and at moderate or low level in other tissues.The expression of FGF 7 was higher in most tissues in multiparous Small Tail Han sheep than in uniparous Sunite sheep,but the difference was not significant (P＞0.05),while the expression of FGF 7 had no obvious differences among 3 genotypes of FecB gene in Small Tail Han sheep.Three genotypes (CC,CT and TT) were found at g.57842893C＞T locus in both uniparous and multiparous sheep,both genotype frequency and allele frequency reached significant difference between uniparous and multiparous sheep (P≤0.05).Population genetic analysis indicated that the g.57842893C＞T locus was at moderate polymorphism (0.25＜PIC＜0.50) in Sunite,Suffolk and Dorper and at low polymorphism (PIC＜0.25) in other sheep breeds;x2 test revealed that the g.57842893C＞T locus was under Hardy-Weinberg equilibrium in Sunite,Suffolk and Dorper sheep (P＞0.05).Association analysis indicated that the g.57842893C＞T locus didn't have significant correlation with the litter size of the first,second or third parity in Small Tail Han sheep (P＞0.05),while the litter size of individuals with CC was higher than those with TT for each parity.Therefore,we concluded that there might be a certain positive correlation between the expression level of FGF 7and the litter size in sheep,although it may not be the key gene for litter size,the g.57842893C＞T locus might provide a basis for litter size trait selection in sheep.关键词
绵羊/FGF 7基因/组织表达/SNP分型/产羔数Key words
sheep/FGF 7 gene/tissue expression/SNP genotyping/litter size分类
农业科技引用本文复制引用
周梅,刘秋月,储明星,曹晓涵,贺小云,孙庆,狄冉,胡文萍,王翔宇,张效生,张金龙..绵羊FGF 7基因组织表达及其多态性与产羔数之间的关系[J].畜牧兽医学报,2018,49(3):525-533,9.基金项目
国家自然科学基金(31772580) （31772580）
国家转基因科技重大专项(2016ZX08009-003-006 （）
2016ZX08010-005-003) （）
国家肉羊产业技术体系专项(CARS-38) （CARS-38）
中央级公益性科研院所基本科研业务费专项(Y2017JC24 （）
2017ywf-zd-13 （）
2013ywf-zd-1 （）
2015ywf-zd-2 （）
2015ywf-zd-8) （）
中国农业科学院科技创新工程(ASTIP-IAS13 （）
CAAS-XTCX2016010-01-03 （）
CAAS-XTCX2016010-03-03 （）
CAAS-XTCX2016011-02-02) （）
宁夏农林科学院科技创新先导资金(DWJLC-2016001) （DWJLC-2016001）
内蒙古自治区科技重大专项 （）
农业科研杰出人才及其创新团队项目 （）
国家万人计划科技创新领军人才项目 （）
天津市科技计划项目(16ZXZYNC00050) （16ZXZYNC00050）
