畜牧兽医学报2018，Vol.49Issue(3)：549-558,10.DOI:10.11843/j.issn.0366-6964.2018.03.011
胰高血糖素样肽-2对羔羊胃肠道重量、瘤胃发酵及小肠上皮发育相关基因表达的影响
Effects of Glucagon-like Peptide-2 on Gastrointestinal Weight, Rumen Fermentation and Expression of Small Intestinal Epithelial Development-Related Genes in Lambs
摘要
Abstract
The objective of this study was to evaluate the effect of glucagon-like peptide-2 (GLP-2) on gastrointestinal weight,rumen fermentation and expression of small intestinal epithelial development-related genes in lambs.In the present study,10 lambs were randomly assigned into 2 groups:control group (0.5％ bovine serum albumin(BSA) in saline,n=5) and GLP-2 group (50 μg· kg-1 BW GLP-2 in0.5％ bovine serum albumin,n=5).At 28 days-old,lambs were subcutaneously injected with BSA or GLP-2 once every 12 hours in the following 14 days.At 42 days-old,all lambs were slaughtered at 2 h after the first injection.Rumen fluid was collected for the analysis of rumen pH and VFA concentration,and the small intestine epithelium was used for quantitative analysis.The results showed that:1) The body weight and gastrointestinal weight of lambs in GLP-2 group were not significantly affected(P＞0.05).GLP-2 treatment had a tendency to increase the weight of jejunum(P=0.083) and ileum(P=0.060).2) GLP-2 injection significantly increased plasma GLP-2 concentration (P =0.025),while there was no significant change in plasma glucose,rumen pH and VFA concentration (P ＞ 0.05) compared with control group.3) Compared with the control group,GLP-2 injection significantly increased the mRNA expression of Cyclin B1 (P=0.001),Cyclin D1 (P=0.044),CDK1 (P=0.028),CDK6 (P=0.016) in duodenum epithelium and CyclinA (P=0.028),Cyclin B1 (P=0.016),CDK1 (P=0.013),CDK4 (P=0.023),CDK6 (P=0.028) in jejunum epithelium,and Cyclin A (P=0.025),Cyclin D1 (P=0.001),CDK2 (P=0.013),CDK4 (P=0.020) in ileum epithelium.4) The mRNA expression of GCG (P=0.036),IGF-1R (P=0.031) in duodenum epithelium and the mRNA expression of GCG (P=0.049),IGF-1 (P=0.027),IGF-1R (P=0.036) and GLP-2R (P=0.011) in jejunum epithelium,and the mRNA expression of GCG (P=0.025),IGF-1 (P=0.029),IGF-1R (P=0.029) and GLP-2R (P=0.032) in ileum epithelium in GLP-2 group were significantly higher than that in the control group.These results suggest that subcutaneous injection of GLP-2 have no significant effect on body weight,gastrointestinal weight and rumen environment in lambs.However,GLP-2 have a tendency to increase jejunum weight and ileum weight,elevate plasma GLP-2 concentration,significantly affect the expression of small intestinal epithelial development-related genes,and promote the development of the small intestine in lambs.These findings will provide new insight into nutritional strategies for promoting the development of small intestine in lambs.关键词
胰高血糖素样肽-2/胃肠道重量/瘤胃发酵/小肠上皮/基因表达/细胞周期蛋白/羔羊Key words
glucagon-like peptide-2/gastrointestinal weight/rumen fermentation/small intestinal epithelium/gene expression/cell cycle protein/lamb分类
农业科技引用本文复制引用
刘理想,孙大明,毛胜勇,刘军花..胰高血糖素样肽-2对羔羊胃肠道重量、瘤胃发酵及小肠上皮发育相关基因表达的影响[J].畜牧兽医学报,2018,49(3):549-558,10.基金项目
国家自然科学基金(31501980) （31501980）
江苏省自然科学基金(BK20150655) （BK20150655）
