畜牧兽医学报2018，Vol.49Issue(3)：597-605,9.DOI:10.11843/j.issn.0366-6964.2018.03.017
河北省猪腹泻相关病毒的检测及猪流行性腹泻病毒S基因遗传变异分析
Detection of Porcine Diarrhea Associated Virus and Genetic Variation Analysis of S Gene of Porcine Epidemic Diarrhea Virus in Hebei Province
摘要
Abstract
To understand the epidemiology of the pigs diarrhea virus in Hebei province,a total of 1 855 clinical samples were collected from April,2016 to February,2017 and subjected to detection of porcine epidemic diarrhea virus (PEDV),transmissible gastroenteritis virus (TGEV) and porcine rotavirus (PoRV) by real-time PCR.Spike (S) genes of nine amplified PEDV were sequenced and analyzed.The results showed that positive rate of diarrhea was 9.70％ and highest in winter and spring seasons.The positive rates of PEDV of total diarrhea samples,piglets diarrhea samples and fattening pigs diarrhea samples were 13.89％,19.28％ and 17.24％,respectively.The phylogenetic analysis of PEDV S genes amino acid sequence showed that the detected 9 S genes from Hebei were distributed in two branches of group Ⅱ.The detail analysis showed several unique amino acid mutations including deletions and insertions in the 9 S genes.These results indicate PEDV infection has fell on piglets but rise on fattening pigs,and the genetic diversity and mutations of S genes were potentially cause alternations of pathogenesis and antigenicity of pandemic PEDV in Hebei province.关键词
猪病毒性腹泻/流行病学调查/猪流行性腹泻病毒/序列分析Key words
porcine viral diarrhea/epidemiological investigation/PEDV/sequence analysis分类
农业科技引用本文复制引用
张若曦,韩庆安,张志,顾文源,刘天驹,李翀,王建昌,李彬,袁万哲,王玉清..河北省猪腹泻相关病毒的检测及猪流行性腹泻病毒S基因遗传变异分析[J].畜牧兽医学报,2018,49(3):597-605,9.基金项目
河北省高层次人才资助项目(2012-28) （2012-28）
河北省畜牧兽医局科技项目(2015-1-01) （2015-1-01）
河北省自然科学基金青年项目(C2017325001) （C2017325001）
河北省高校百名优秀创新人才支持计划(Ⅲ) (SLRC2017039) （Ⅲ）
