畜牧兽医学报2018，Vol.49Issue(3)：614-619,6.DOI:10.11843/j.issn.0366-6964.2018.03.019
检测兔出血症病毒抗体胶体金试纸条的研制及初步应用
Development and Preliminary Application of a Colloidal Gold Test Strip for Rabbit Hemorrhagic Disease Virus Antibody
摘要
Abstract
A colloidal gold test strip for rapid detection of RHDV antibody was developed based on colloidal gold immunochromatographic assay.The colloidal gold particles were labeled with recombinant RHDV VP60 protein.The anti-VP60 monoclonal antibody A3C and Staphylococcus aureus protein A (SPA) were respectively coated on the control line (C line) and test line (T line).The strip was optimized by detecting strong positive,positive,weak positive and negative serum,and its qualities and coincidence rate of HI test were verified.The results indicated that the T and C lines were the same color when the positive serum was detected.The T line was darker than the C line when the strong positive serum was detected.The T line was lighter than the C line when the weak positive serum was detected.Compared with HI test,the coincidence rates of strong positive,positive,weak positive,negative serum were 93.75％,88.75％,91.25％,95.00％,respectively,while the total coincidence rate was 91.54％.The colloidal gold strip has specificity,sensitivity,repeatability and stability,and provide a tool for epidemiological survey and antibody level monitoring.关键词
兔病毒性出血症/兔出血症病毒/VP60/胶体金试纸条/抗体Key words
rabbit hemorrhagic disease/rabbit hemorrhagic disease virus/VP60/colloidal gold strip/antibody分类
农业科技引用本文复制引用
魏后军,范志宇,王芳,宋艳华,胡波,仇汝龙,陈萌萌,徐为中,薛家宾..检测兔出血症病毒抗体胶体金试纸条的研制及初步应用[J].畜牧兽医学报,2018,49(3):614-619,6.基金项目
现代农业产业技术体系建设兔体系病毒病预防与控制岗位(CARS-44-C-1) （CARS-44-C-1）
公益性行业(农业)科研专项(201303046) （农业）
