畜牧与兽医2018，Vol.50Issue(3)：53-58,6.
腹泻仔猪肠道沙门氏菌耐药性研究
Study on drug resistance of intestinal Salmonella in diarrhea piglets
摘要
Abstract
In order to understand the drug-resistance phenotype and genotype of Salmonella in diarrhea piglets of large-scale farms in Guizhou Province,the intestinal samples of diarrhea piglets in 6 large-scale pig farms in Guizhou Province were collected to isolate and identify Salmonella strains.Then the drug-resistance phenotype and genotype of these isolates were analyzed using the susceptibility test and PCR.In total,21 strains of Salmonella were isolated and identified from the samples.The drug-resistance test showed that the rates of resistance to drugs of the identified Salmonella were in the descending order of kanamycin,sulfamethoxazole and norfloxacin.All the isolates resisted against multiple antibiotics,and five of the isolates resisted against more than 8 different antibiotics.Furthermore,the results of the present study showed that the coincidence rate between β-1actam-resistance genotype and phenotype was as high as 100％.In the of quinolone resistance,the coincidence rate was 62.5％,in aminoglycoside resistance,the coincidence rate was 73.3％,and in sulfanilamide resistance,the coincidence rate was 81.8％.The present study provides a theoretical support for rational use of drugs in controlling Salmonella infection in piglets.关键词
沙门菌/分离鉴定/耐药性/耐药基因Key words
Salmonella/isolation and identification/drug resistance/drug resistance gene分类
农业科技引用本文复制引用
刘丽娟,马光强,杨琦,张双翔,王伟,胡兴义,张海,王开功,周碧君..腹泻仔猪肠道沙门氏菌耐药性研究[J].畜牧与兽医,2018,50(3):53-58,6.基金项目
贵州省科学技术厅农业攻关项目(黔科合NY[2015]3009-1号) （黔科合NY[2015]3009-1号）
贵州省动物疫病防控与兽医公共卫生保障科技创新人才团队(黔科合人才团队[2015]4016号).贵州省农业委员会科技计划项目“贵州省2015年重大动物疫病省级定点监测”[2015-01号] （黔科合人才团队[2015]4016号）
