畜牧与兽医2018，Vol.50Issue(3)：77-81,5.
绵羊肺炎支原体P109'蛋白的分子特征及抗原性分析
Molecular characterization and antigenicity of Mycoplasma ovipneumoniae P109'protein
摘要
Abstract
To study the structural characteristics and immunogenicity of P109'protein of Mycoplasma ovipneumoniae,bioinformatic analysis was performed and a truncated fragment ofpl09'was amplified by PCR and cloned into a pET-32a (+) vector so as to construct prokaryotic expression plasmid.The reactionogenicity of the protein was evaluated by Western-blot.The results showed that p109'was homologous with the genes that encoded the hypothetical protein in Mycoplasma dispar,the p102 gene in Mycoplasma hyopneumoniae and the gene that encoded the P102-1ike protein.The recombinant protein was successfully expressed in Escherichia coli BL21 (DE3) as an included body.The purified recombinant protein reacted with the goat serum against M.ovipneumoniae,suggesting that the P109'protein has a good reactionogenicity.The results obtained in this study provided useful information for future investigation of the functions of the M.ovipneumoniae protein P109'.关键词
绵羊肺炎支原体/P109'蛋白/原核表达/免疫原性Key words
Mycoplasma ovipneumoniae/P109'protein/prokaryotic expression/immunogenicity分类
农业科技引用本文复制引用
马媛,管礼麟,刀筱芳,杨发龙..绵羊肺炎支原体P109'蛋白的分子特征及抗原性分析[J].畜牧与兽医,2018,50(3):77-81,5.基金项目
国家重点研发计划(2016YFD0500907) （2016YFD0500907）
四川省学术与技术带头人培养支持经费 （）
西南民族大学研究生创新型科研项目(CX2017SZ055) （CX2017SZ055）
