畜牧与兽医2018，Vol.50Issue(3)：87-92,6.
荧光定量RT-PCR方法检测HP-PRRSV JXA1分离株在藏猪、藏梅猪和大约克夏猪体内的动态分布
Dynamic distribution of HP-PRRSV JXA1 isolate in Tibetan, ZangMei black and Yorkshire pigs after infection tested by real-time quantitative PCR
摘要
Abstract
In order to investigate viral distribution in PRRSV-infected Tibetan,ZangMei black and Yorkshire pigs,we used 1 mL 4× 105 TCID50/ml of HP-PRRSV isolate to infect Tibetan,ZangMei black and Yorkshire pigs (15 for each breed) and measured viral RNA loads in each of the fifteen tested organs of the pigs on 0,4,7,14 days after PRRSV challenge.The 15 tested organs were heart,liver,spleen,lung,kidney,brain,small intestine,mesenteric lymph node,groin lymph node,submandibular gland,mandibular lymph node,thyroid,thymus,bronchial lymph nodes and tonsil.The results were that viral RNA was identified in all the fifteen organs of ZangMei black and Yorkshire pigs,but not in the heart,liver,spleen,kidney,small intestine,submandibular gland and thyroid of the Tibetan pigs during infection.The maximum viral RNA loads in the organs of the three pig breeds 4,7,and 14 days after infection were evidently different,with the highest loads in the amygdala,inguinal lymph nodes and lymph nodes of lower jaw of the Tibetan pigs,in the spleen and lung of ZangMei black pigs,and in the thymus,lung and lower jaw lymph of the Yorkshire pigs.To sum up,HP-PRRSV infection results in different distribution patterns of virus in the three pig breeds as a whole and in different organs of individual breeds of the pigs.关键词
JXA1分离株/藏猪/藏梅猪/大约克夏猪/荧光定量RT-PCR/动态分布Key words
JXA1 isolate/Tibetan pig/ZangMei black pig/Yorkshire pig/real-time quantitative PCR/dynamic distribution分类
农业科技引用本文复制引用
康润敏,姬高升,周泷,殷明郁,曾凡亚,王红宁..荧光定量RT-PCR方法检测HP-PRRSV JXA1分离株在藏猪、藏梅猪和大约克夏猪体内的动态分布[J].畜牧与兽医,2018,50(3):87-92,6.基金项目
四川公益性科研院所基本科研项目(2016YSKY0144) （2016YSKY0144）
四川省科技支撑计划(2014NZ0092,16ZC2850) （2014NZ0092,16ZC2850）
国家生猪产业技术体系(CARS-36) （CARS-36）
四川省生猪现代产业链关键技术研究与集成示范(2012GZ0001-) （2012GZ0001-）
国家现代农业体系四川创新团队建设(sccxtd-004) （sccxtd-004）
四川省畜禽育种攻关(2016NZ0108) （2016NZ0108）
