畜牧与兽医2018，Vol.50Issue(3)：93-96,4.
鹅圆环病毒福建株全基因组的克隆及序列分析
Cloning and sequencing the complete genome of the Fujian strain of goose circovirus
摘要
Abstract
In order to characterize the genome of the goose circovurs isolated in Fujian (the FJ2016 strain),a reverse primer was designed to obtain the genome sequence of the FJ2016 strain by means of PCR amplification in a goose in stunted growth.The results showed that the genome of the FJ2016 strain was 1821 bp long,its replicated protein was 882 bp in length encoding 293 amino acids,and its anti-genetic protein was 753 bp long encoding 250 amino acids.In the perspective of genetic evolution of the genome,GoCV could be divided into 2 branches:GoCV-1 and GoCV-2 branch,with the FJ2016 strain being in the GoCV-1 branch.Compared with the nucleotide homology,the FJ2016 strain exhibited the highest homology with yk2 at 98.2％,but lower with the Greylag goose circovirus 2GK strain at 82.4％,and even lower with Cygnusolor circovirus at 73.7％ (below 80％).The FJ2016 strain shared 97.3％ to 98.2％ of homology with the goose circoviruses in the GoCV-1 branch,and 93.1％ to 93.5％ with the goose circoviruses in the GoCV-2 branch.This study was the first report on circovirus infection in geese in Fujian Province.关键词
鹅圆环病毒/基因组/克隆/序列分析Key words
goose circovirus/genome/clone/sequence analysis分类
农业科技引用本文复制引用
吴方达..鹅圆环病毒福建株全基因组的克隆及序列分析[J].畜牧与兽医,2018,50(3):93-96,4.基金项目
福建省优良蛋鸭品种与设施化养殖创新产业化工程项目 （）
