畜牧与兽医2018，Vol.50Issue(3)：103-106,4.
蓝狐源肺炎克雷伯菌的鉴定与药敏试验
Isolation, identification and drug susceptibility of Klebsiella pneumoniae isolated from blue fox
摘要
Abstract
In August 2016,a number of blue foxes on a farm in Changli County,Hebei Province suffered from dyspnea and acute death.To identify the pathogenic bacterium in the case,one strain was isolated from the tissues of the liver,heart blood and lung of the dead fox.The isolate received characteristics training,morphological observation and biochemical test and was found to be Klebsiella pneumoniae.16S rRNA sequence analysis showed that the bacterium was 98％～ 99％ homologous with nine strains of Klebsiella accessed in the Genbank.An animal regression test proved the strain with strong pathogenicity in mice resulting in LD50 being 3.2× 106 CFU/mL.Drug susceptibility testing showed that the strain of the pathogenic bacterium was sensitive to enrofloxacin,ciprofloxacin,spectinomycin and ceftriaxone but of multiantibiotic resistance to other drugs.关键词
蓝狐/肺炎克雷伯菌/分离鉴定/药敏试验Key words
blue fox/Klebsiella pneumoniae/isolation and identification/drug susceptibility test分类
农业科技引用本文复制引用
李巧玲,贾青辉,吉志新,肖丽荣,张文举,张召兴,吴同垒,张志强,史秋梅,李蕴玉,李佩国..蓝狐源肺炎克雷伯菌的鉴定与药敏试验[J].畜牧与兽医,2018,50(3):103-106,4.基金项目
河北省科技厅科技支撑计划(16236605D-3) （16236605D-3）
科技部星火计划(2015GA620002) （2015GA620002）
河北省科技厅科技支撑计划(17227302D) （17227302D）
石家庄市科技局项目(171500953A) （171500953A）
秦皇岛市科技局项目(201602A046),秦皇岛市科技局项目(201702B001),河北省科技厅农业关键共性技术攻关专项项目(16227525) （201602A046）
