畜牧与兽医2018，Vol.50Issue(3)：107-111,5.
中药提取物恢复耐药大肠杆菌对氨基糖苷类药物敏感性的研究
Restoring the sensitivity of drug-resistant Escherichia coil to aminoglycoside antibiotics using Chinese herbal extracts
摘要
Abstract
In order to detect the susceptibility of the extracts of five Chinese herbs Galla Chinensis,Scutellariae Radix,Coptidis Rhizoma,Artemisiae Argyi Folium and Houttuyniae Herba to restore the sensitivity of drug-resistant Escherichia coli to aminoglycoside antibiotics,the antibacterial properties of the experimental strains were eliminated by 1/2MIC Chinese herbal medicine,the drug-resistant colonies were screened by photographic cuhure,the rate of drug-resistant elimination and MIC eliminated were observed,and the expression of the plasmid and drug resistance gene Rmt B were detected.The results showed that the five Chinese herbal extracts had a certain inhibitory effect on the drug-resistant Escherichia coli.48 h treatment of the experimental strains by 1/2 MIC resulted in the elimination rates of 20.67％ by gallnut,21.33％ by Scutellaria baicalensis,17.33％ by Coptis chinensis,16.67％ by Iziphus jujuba and 9.33％ by Houttuynia cordata,respectively.The MIC values of the elimination for GENA,KAN,AMK and NEO were from high resistance (＞512 μg/mL) to sensitivity (2 ～4 μg/ml).The five Chinese herbal extracts could not remove the drug resistance plasmids,but they could drop the drug-resistant gene Rmt B.To conclude,the extracts of the five Chinese herbs could restore drug sensitivity by dropping the major drug-resistant genes from the bacteria that are highly resistant to aminoglycosides.关键词
中药/耐药性/敏感性/氨基糖苷类药物Key words
traditional Chinese medicine/drug resistance/sensitivity/aminoglycoside antibiotics分类
医药卫生引用本文复制引用
刘金平,吕世明,谭艾娟,杨可,寇宏,王想,张顺然,杨安美..中药提取物恢复耐药大肠杆菌对氨基糖苷类药物敏感性的研究[J].畜牧与兽医,2018,50(3):107-111,5.基金项目
国家自然科学基金项目(31760749)“sul基因在猪源大肠杆菌和人源嗜麦芽寡养单胞菌之间转移频率研究”,贵州大学研究生创新基金项目“中药降低大肠杆菌对氨基糖苷类药物耐药性的研究”(研农2017006) （31760749）
