中国医疗设备2018，Vol.33Issue(4)：17-21,5.DOI:10.3969/j.issn.1674-1633.2018.04.005
一种捕获尿液中膀胱癌细胞的新型微流控芯片的设计 新型微流控芯片的设计
Design of a Microfluidic Chip for Bladder Cancer Cell Capturing in Urine
摘要
Abstract
Bladder cancer mainly occurred on the bladder mucosa, and is the most common malignancy of the urinary system known as its high morbidity and mortality. However, many weaknesses and shortcomings still exist in current clinical diagnosis for bladder cancer, such as invasive detection, low specificity and sensibility. We proposed and designed a new type of microfluidic chip used for the detection of bladder cancer. Polydimethylsiloxane was used as the material of the microfluidic chip for production utilizing cast molding method. Specific antibody was fixed onto a glass slide after silanization modification and used to capture target cells with cylindrical micropattern which could slow down the cell velocity. Adopting the microfluidic chip, bladder cancer cells were captured from the urine sample of patients and identified by specific fluorescent antibody. The results showed that the microfluidic chip could specifically capture bladder cancer cells from urine of patients. This study has successfully established the microfluidic chip which detects bladder cancer noninvasively and provides new ideas and powerful platform for detection and diagnosis of bladder cancer.关键词
微流控芯片/膀胱癌细胞/细胞抓捕/免疫荧光Key words
microfluidic chip/bladder cancer cell/cell capture/immunofluorescence分类
医药卫生引用本文复制引用
耿春阳,李驰宇,李雅楠,任海军,翟晓峰,刘波..一种捕获尿液中膀胱癌细胞的新型微流控芯片的设计 新型微流控芯片的设计[J].中国医疗设备,2018,33(4):17-21,5.基金项目
国家自然科学基金(31670867 （）
31670961). （）
