疑难病杂志2018，Vol.17Issue(4)：395-399,404,6.DOI:10.3969/j.issn.1671-6450.2018.04.017
小鼠正畸牙移动及牙周组织改建中Sema3A的作用
The effect of Sema3A on the periodontal tissue remodeling during orthodontic tooth movement of mice
摘要
Abstract
Objective To discuss the expression of Sema3A in orthodontic tooth movement and periodontal tissue re-modeling in mice,and clarify the specific temporal and spatial regularity of Sema3A expression during the process. Methods Thirty-six wildtype(C57BL/6)mice were selected to establish the orthodontic tooth movement model. The loading group was applied by 10-15 grams force between the first molar and incisor teeth in the left side of maxillary,while the control group was applied in the right side. 9 mice were killed on the 1st day,the 7th day and the 14th day respectively. 3 of them were made into paraffin tissue sections to observe morphology with immunohistochemical staining;3 of them were made into hard tissue sections(without decalcification) to show calcein bone deposition with Masson staining;3 of them were analyzed the express level of Sema3A gene with Western blotting. Results (1) Tooth movement was observed in all the experimental rats. It showed that the modeling establishment was successful.(2) In the control group,the periodontal tissue configuration was nor-mal,there was no bone absorption or bone formation and other changes,and osteoblast is extremely rare. On the first day in the loading group,there was no obvious change in the periodontal tissue. On the 7th day,fiber membrane disorder was appar-ent. The periodontal ligament narrowing and a large number of osteoblast were visible. But on the 14th day, the morphology returned to normal with less osteoblast distribution.(3) Immunohistochemical staining showed the Sema3A expression was con-sistent negative in control group. In loading group, Sema3A expression was weak positive on the 1st day, then it rose to a strong positive expression on the 7th day, but it returned to weak positive on the 14th day.(4) Western blotting detection showed that there were Sema3A protein prints in two groups. Semi-quantitative analysis showed that there was no Sema3A pro-tein expression fluctuation in control group,which meant in low level expression. In loading group,Sema3A protein expression was low on the 1st day. Sema3A protein expression increased to the peak(12.83 ± 1.63) on the 7th day,which was signifi-cantly higher than the control group (P<0.05). On the 14th day,Sema3A protein expression was restored to a similar level as the control group. Conclusion In the movement of mice orthodontic tooth,Sema3A may involve in the remodeling of the periodontal tissue,and its expression has certain regularity of the temporal variation. In orthodontic bone remodeling process, Sema3A can promote bone formation. It has a positive protective effect on alveolar bone remodeling.关键词
正畸牙移动/牙周组织改建/骨改建/Sema3A/小鼠Key words
Orthodontic tooth movement/Periodontal tissue remodeling/Bone remodeling/Sema3A引用本文复制引用
陈钰文,白秋野,胡丽华,沈丹阳,阎秀林..小鼠正畸牙移动及牙周组织改建中Sema3A的作用[J].疑难病杂志,2018,17(4):395-399,404,6.基金项目
辽宁省教育厅科学技术计划项目(LK201607) （LK201607）
