中国病理生理杂志2018，Vol.34Issue(4)：764-768,5.DOI:10.3969/j.issn.1000-4718.2018.04.030
一种建立小鼠2型糖尿病心肌病模型的方法
A method of establishing type 2 diabetic cardiomyopathy model in mice
摘要
Abstract
AIM:To establish a mouse model of type 2 diabetic cardiomyopathy induced by sustained high-fat diet(HFD)feeding and single intraperitoneal injection of streptozotocin(STZ).METHODS: The 5 ~6-week-old C57BL/6J mice were randomly divided into 2 groups(n=20 per group): the mice in control group received a sustained regular diet;the mice in HFD+STZ group received a sustained HFD and were intraperitoneally injected with STZ(100 mg/kg)at the 5th week.The body weight and blood glucose were measured at 0,5,6,11 and 16 weeks.The mice in control group and HFD +STZ group were analyzed with echocardiography,HE staining,ELISA and immunohistochemistry at the 11th and 16th weeks.RESULTS:The body weight of the mice in HFD +STZ group was higher than that in control group(P<0.05)during each modeling period,and was slightly decreased 1 week after STZ injection.The blood glucose of the mice in HFD+STZ group was higher than 13.89 mmol/L 1 week after STZ injection.The serum insulin of the mice in HFD+STZ group was lower than that in control group(P<0.05)at the 11th week and the 16th week.Echocardio-graphy, HE staining and immunohistochemistry analysis showed that the mice in HFD +STZ group had mild ventricular dysfunction and cardiomyocyte hypertrophy, and cardiomyocyte area and apoptosis rate were obvious higher than those in control group(P<0.05)at the 16th week,while these indicators were not obviously changed in HFD +STZ group com-pared with control group at the 11th week.CONCLUSION:The sustained HFD feeding and single intraperitoneal injec-tion of STZ successfully establish a mouse model of type 2 diabetic cardiomyopathy.关键词
糖尿病心肌病/小鼠模型/高脂饮食/链脲佐菌素/细胞凋亡Key words
Diabetic cardiomyopathy/Mouse model/High-fat diet/Streptozotocin/Apoptosis分类
医药卫生引用本文复制引用
王金鑫,段鹏,朱庆磊..一种建立小鼠2型糖尿病心肌病模型的方法[J].中国病理生理杂志,2018,34(4):764-768,5.基金项目
国家自然科学基金资助项目(No.81570349) （No.81570349）
