局解手术学杂志2018，Vol.27Issue(1)：16-20,5.DOI:10.11659/jjssx.09E017040
雌激素及其受体与IL-17在HPV16持续感染阶段的表达研究
Expression of IL-17 and estrogen and its receptor in HPV16 continuously infected patients
摘要
Abstract
Objective To understand the role of estrogen,estrogen receptor and IL-17 in human papillomavirus (HPV) infection and clearance.Methods We selected the clinic or the hospitalized patients in People' s Hospital of Pudong New District hospital with HPV negative or single-HPV16 positive during the period of August 2014 to January 2017.Cervical exfoliated cells were harvested by Thinprep cytologic test (TCT).The reverse spot hybridization technique was carried out for HPV subtype analysis.RT-PCR was employed for HPV16 DNA viral load.The levels of E2 and IL-17 were detected by ELISA.Immunohistochemical was used to detect ER expression.The experiments were repeated every three months.According to the difference in the outcome of human papilloma virus 16 infection,the experiment was divided into three groups.The first group was HPV negative,which was negative for one year.The second group was HPV16 positive,but within one year the difference was gradually cleared.The third group was HPV16 positive,but the HPV16 persistently existed after one year.Results The results of the last test were compared with the first test results.We found that There was no significant difference among the three groups in the expression of E2 and ER.Also,there was no significant difference between the HPV negative group and the HPV16 persistently infected group in variation of IL-17 concentration.But the difference between the HPV16 cleared group and the anyone of the two groups above was significantly.The variation of IL-17 concentration was significantly higher in the HPV16 cleared group.Conclusion In normal cytology,and only HPV16 infected stage,endogenous E2 and ER of cervix may not play a role in HPV infection,or they may have little effect on HPV infection.Local immune factor IL-17 plays an important role in the process of HPV16 removal,and the increase of IL-17 concentration can help to eliminate HPV16.关键词
人乳头瘤病毒16型/持续感染/雌激素/雌激素受体/白介素-17Key words
human papilloma virus-16/persistent infection/estrogen/estrogen receptor/IL-17分类
医药卫生引用本文复制引用
周爱枝,段迎春,张蕾,戴晓莉,杨玉梅,任青..雌激素及其受体与IL-17在HPV16持续感染阶段的表达研究[J].局解手术学杂志,2018,27(1):16-20,5.基金项目
上海市卫生和计划生育委员会科研课题资助项目(201440069) （201440069）
