中国临床药理学与治疗学2018，Vol.23Issue(2)：126-131,6.DOI:10.12092/j.issn.1009-2501.2018.02.002
酸枣仁皂苷A对脂多糖诱导小胶质细胞活化的影响及神经保护作用
Jujuboside A attenuates LPS-induced microglial activation and provides neuroprotection
摘要
Abstract
AIM:To investigate the inhibitory effects of Jujuboside A (Ju A) on LPS induced inflammation mediators in BV-2 microglial cells,and the neuroprotective effect of Ju A in neuronal injury.METHODS:BV-2 ceils were pre-treated with Ju A,and then stimulated by LPS.The cell viabilities were measured by MTT assay,the levels of NO were measured by Griess assay,the concentrations of TNF-α,IL-1β were detected by using ELISA,the contents of ROS were detected by DCFH-DA probe method,and the effect of BV-2 conditioned medium on survival rate of HT-22 cells was detected by MTT.RESULTS:Ju A had no significant effect on cell viability within tested concentrations.Ju A significantly inhibited the production of NO,TNF-α,IL-1β and ROS.BV-2 conditioned medium intervened by Ju A inhibited the neuroinflammation on HT-22 hippocampal neuron cells.CONCLUSION:Ju A inhibits LPS-induced microglia activation and neuroinflammation,which mediates its neuroprotecrive effect on neuronal injury.关键词
酸枣仁皂苷A/小胶质细胞/海马神经元细胞/神经炎症/神经保护Key words
Jujuboside A/microglial cell/hippocampal neurons/neuroinflammation/neuroprotective分类
医药卫生引用本文复制引用
张睿,李冰洁,李庆林..酸枣仁皂苷A对脂多糖诱导小胶质细胞活化的影响及神经保护作用[J].中国临床药理学与治疗学,2018,23(2):126-131,6.基金项目
国家自然科学基金(30973894) （30973894）
