中国临床药理学与治疗学2018，Vol.23Issue(2)：132-137,6.DOI:10.12092/j.issn.1009-2501.2018.02.003
肾衰宁颗粒对糖尿病肾病大鼠肾组织NF-κB及PPAR-γ表达的影响
Effects of Shenshuaining granules on the expression of NF-κB and PPAR-γ in kidney tissue of diabetic nephropathy rats
摘要
Abstract
AIM:To study the effects of Shenshuaining granules on the expression of nuclear factor kappa B (NF-κB) and peroxisome proliferator activated receptor γ (PPAR-γ) in kidney tissue of diabetic nephropathy (DN) rats.METHODS:The DN rats model were induced by high-fat diet combined with intraperitoneal injection of streptozotocin (35 mg/kg).Forty DN rats model were randomly divided into control group (same volume 0.9％ NaCl),Shenshuaining (SSN) group (SSN granules 2 g/kg),saxagliptin group (saxagliptin tables 0.6 mg/kg),SSN and saxagliptin group (SSN granules 2 g/kg + saxagliptin tables 0.6 mg/kg) intragastric administration once per day,for 8 weeks.The renal pathological changes were evaluated by HE staining,the fasting blood glucose (FBG) were tested by glucose meter,glycosylated hemoglobin (HbA1 c) were tested by glycated hemoglobin meter,serum creatinine (Scr),urea nitrogen (BUN) and serum Cystatin C (Cys-C) were tested by automatic biochemical analyzer,the levels of tumor necrosis factor α (TNF-α),intercellular adhesion factor-1 (ICAM-1) and monocyte chemotactic protein-1 (MCP-1)were detected by ELISA method,and the expression of NF-κB and PPAR-γwere detected by immunohistochemical method.RESULTS:The glomerular capillary structure was damaged and fibrosis in the control group,and capillary lumen was shown stenosis and lipid deposition,the renal interstitial was also shown blood vessel growth.The glomerular lesions were improved in experimental group,and glomerular and capillary structures were relatively intact.The levels of FBG,HbAlc,Scr,BUN,Cys-C,TNF-α,ICAM-1 and MCP-1 in experimental group were significantly lower than those of control group,the differences had statistical significance (P ＜0.05).The expression of NF-κB in experimental group was significantly lower than that of the control group,and the expression of PPAR-γ was higher than that of control group,the differences had statistical significance (P ＜ 0.05).CONCLUSION:Shenshuaining granules can improve the renal glomerulus and capillary structure for diabetic nephropathy rat,reduce blood sugar,protect renal function,alleviate inflammation,relieve kidney disease,the mechanism may be related with decreasing NF-κB expression and increasing PPAR-γ expression in glomerular tissue.关键词
肾衰宁颗粒/糖尿病肾病/肾功能/炎症反应/核因子-κB/过氧化物酶体增殖体激活受体γKey words
Shenshuaining granules/diabetic nephropathy/renal function/inflammatory response/nuclear factor kappa B/peroxisome proliferator activated receptor γ分类
医药卫生引用本文复制引用
姜莉莉,毛利民,胡思佳,彭应枝..肾衰宁颗粒对糖尿病肾病大鼠肾组织NF-κB及PPAR-γ表达的影响[J].中国临床药理学与治疗学,2018,23(2):132-137,6.基金项目
浙江省医药卫生科技计划项目(2013KYB002) （2013KYB002）
