中国临床药理学与治疗学2018，Vol.23Issue(2)：138-142,5.DOI:10.12092/j.issn.1009-2501.2018.02.004
乳复宁颗粒调节乳腺增生症家兔激素水平以及VEGF蛋白表达变化的研究
Effects of Rufuning granules on hormone levels and the expression of VEGF protein in mammary gland hyperplasia rabbits
摘要
Abstract
AIM:To investigate the mechanism and the effects of Rufuning granules on improving the symptom of mammary gland hyperplasia(MGH).METHODS:Forty-eight rabbits were randomly divided into six groups (n =8),normal control group,model group,Rupixiao tablets group,Rufuning-high dose,medium dose and low dose groups.The model of MGH was induced by injecting estradiol benzoate in unpregnant female rabbits.Rabbits in the normal control received saline injection only.The breast diameter and nipple height of rabbits were determined.The contents of prolactin (PRL),estradiol(E2) and progesterone (P) in serum in each group were detected.Pathologic changes in morphology due to MGH in rabbits were observed by microscopy.Meanwhile,the expression of VEGF protein in rabbit breast tissue was determined.RESULTS:The breast diameter and nipple height of rabbits were reduced after treatment in high and medium doses of Rufuning granules group (P ＜ 0.05).The contents of PRL and E2 in serum were reduced (P ＜ 0.05)while those of progesterone in serum were improved (P ＜ 0.05).The performance of histology breast hyperplasia was significantly improved.The number of mammary gland flocculus and lobular gland bubble and lumen expansion were reduced in rabbit mammary.Mammary gland hyperplasia of interstitial fibrous connective tissue was also significantly alleviated as compared with those in model group.Meanwhile,VEGF over-expression in breast tissue was reduced.CONCLUSION:Rufuning granules may improve symptom of MGH by adjusting the ratio of estrogen and progesterone and reducing VEGF over-expression.关键词
乳复宁颗粒/乳腺增生/雌二醇/孕酮/VEGF蛋白Key words
Rufuning granules/mammary gland hyperplasia/estradiol/progesterone/VEGF分类
医药卫生引用本文复制引用
李睿,孔艳,刘增辉,罗胜勇..乳复宁颗粒调节乳腺增生症家兔激素水平以及VEGF蛋白表达变化的研究[J].中国临床药理学与治疗学,2018,23(2):138-142,5.基金项目
安徽省自然科学基金面上项目(1608085MH161) （1608085MH161）
