中国临床药理学与治疗学2018，Vol.23Issue(2)：143-147,5.DOI:10.12092/j.issn.1009-2501.2018.02.005
樟芝分级沉淀的多糖组分对链脲佐菌素构建的糖尿病小鼠的降糖作用及体外抑制α-糖苷酶活性的研究
Inhibition of alpha glucosidase activity in vitro and the hypoglycemic effect of antrodia camphorata polysaccharide for diabetic mice construction by streptozotocin
摘要
Abstract
AIM:To investigate the hypoglycemic effect and inhibition of alpha glucosidase activity in vitro of three polysaccharide obtained from ethanol fractionation precipitation on diabetic mice constructed with streptozotocin (STZ).METHODS:Three polysaccharides were obtained by fractionation and precipitation of total polysaccharides with ethanol.The diabetic mice were constructed by intraperitoneal injection of STZ and divided into control group,model group,drug group (divided into three groups,three kinds of polysaccharides).Fourteen days after drug intervention,the fasting blood glucose (FBG),insulin (FINS),glucagon (GC),expression of insulin resistance index (HOMA-IR),SOD,MDA and GSH-Px in pancreatic islets of mice were detected.RESULTS:Three kinds of polysaccharides had hypoglycemic effects at different degrees,and their effects were related to the expression of insulin and glucagon.Polysaccharides also interfered with the expression of SOD,MDA and GSH-Px in islet tissues at different degrees.In vitro studies showed that polysaccharides significantly inhibited alpha glucosidase and the effects were dosedependent.CONCLUSION:The polysaccharide has significant therapeutic effect on diabetic mice,but the effects of different components of polysaccharides are different,and the mechanism is diverse.Antrodia camphorate polysaccharide is a promising polysaccharide drug,which deserves further study and development.关键词
多糖/糖尿病小鼠/α-糖苷酶Key words
polysaccharides/diabetic mice/alpha glucosidase分类
医药卫生引用本文复制引用
杨毅,官俏兵,张晓玲,郭丽,王琰萍,韩晨阳..樟芝分级沉淀的多糖组分对链脲佐菌素构建的糖尿病小鼠的降糖作用及体外抑制α-糖苷酶活性的研究[J].中国临床药理学与治疗学,2018,23(2):143-147,5.基金项目
嘉兴市科技计划项目(2015AY23021,2017BY18023) （2015AY23021,2017BY18023）
浙北专病中心建设项目[浙卫发2015年(21)号] （21）
