中国临床药理学与治疗学2018，Vol.23Issue(2)：148-153,6.DOI:10.12092/j.issn.1009-2501.2018.02.006
IL-17A与脂多糖诱导的小鼠急性肺损伤早期反应的关系
Study on interleukin-17A during early inflammatory response in mice with LPS-induced acute lung injury
摘要
Abstract
AIM:To observe the dynamic changes of IL-17A in lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice,and to assess the correlation of IL-17A during early inflammatory response of ALI.METHODS:Mice were used to establish the ALI group and its control group by intratracheal administration of LPS and normal saline,respectively.The level of IL-17A in bronchoalveolar lavage fluid (BALF) and MCP-1 in serum,histopathological changes in lung tissue,lung wet weight/dry weight ratio (W/D),the expression of intercellular adhesion molecule 1 (ICAM-1) and the myeloperoxidase (MPO) activity in lung homogenates would be tested after modeling for 3,8,14,24 h.RESULTS:Compared with the controls,the level of IL-17A in BALF increased (P ＜ 0.05) and the level of MCP-1 in serum increased significantly (P ＜0.01),and as time went by,the three above decreased gradually,and the histological examination of lung showed inflammatory changes,and W/D ratio,expression of ICAM-1,MPO activity aggravated gradually in the ALI group (P ＜ 0.05).CONCLUSION:The level of IL-17A in BALF increased significantly during early ALI,and this shows that IL-17A may play an important role in the progress of early inflammatory response in LPS-induced ALI.关键词
急性肺损伤/脂多糖/白介素17AKey words
acute lung injury/lipopolysaccharide/IL-17A分类
医药卫生引用本文复制引用
郑爽,付秦磊,周玲萍,彭传鹏,徐红蕾..IL-17A与脂多糖诱导的小鼠急性肺损伤早期反应的关系[J].中国临床药理学与治疗学,2018,23(2):148-153,6.基金项目
温州市科技局科研基金(Y20140680) （Y20140680）
浙江省卫生厅科研基金(2015RCB018) （2015RCB018）
浙江省自然科学基金(LY12H01001) （LY12H01001）
