中国麻业科学2018，Vol.40Issue(1)：1-7,46,8.
苎麻BnNRT1.1基因的克隆及表达特性研究
Cloning and Expression Characteristics of BnNRT1.1 Gene in Ramie
摘要
Abstract
NRT1.1 (nitrate transporter 1.1) plays a very important role in the absorption and utilization of nitrate in plants.To identify the mechanism on ramie may be helpful to improve the nitrogen use efficiency of ramie.In this study,the full-length cDNA sequence of ramie BnNRT1.1 gene was cloned,containing 1869 bp,open reading frame (1776 bp) and encoding 591 amino acids.The results of bioinformatics analysis showed that the protein molecular weight of BnNRT1.1 is 65.25 kD and the isoelectric point is 8.85,which is a hydrophobic protein with 12 transmembrane domains and 15 phosphorylation sites.It has important functions in signal transduction and metabolism of nitrogen translocation.Phylogenetic analysis showed that BnNRT1.1 gene of ramie is homologous with Prunus avium,Pyrus x bretschneideri and Morus notabilis.Tissue specific expression characteristics analysis showed that BnNRT1.1 was mainly expressed in Ramie roots,but it was relatively low in stems and leaves.Nitrate induction treatment showed that the expression of BnNRT1.1 in Longtan dama with high nitrogen use efficiency was higher than that in Qingpi ganma with low nitrogen use efficiency and the expression reached the maximum after treatment three days in different varieties.This study provides a molecular biological basis for the absorption,translocation and regulation of nitrate in ramie in order to improve the nitrogen use efficiency of Ramie.关键词
苎麻/BnNRT1.1/生物信息学分析/表达分析/氮素利用率Key words
ramie/BnNRT1.1/bioinformatics analysis/expression analysis/nitrogen use efficiency分类
农业科技引用本文复制引用
侯美,高钢,朱爱国,陈平,陈继康,陈坤梅,熊和平,喻春明..苎麻BnNRT1.1基因的克隆及表达特性研究[J].中国麻业科学,2018,40(1):1-7,46,8.基金项目
国家麻类产业技术体系(CARS-19-E03) （CARS-19-E03）
中国农业科学院科技创新工程(ASTIP-IBFC05) （ASTIP-IBFC05）
