中国输血杂志2018，Vol.31Issue(1)：37-40,4.DOI:10.13303/j.cjbt.issn.1004-549x.2018.01.010
多色探针熔解曲线法应用于别嘌呤醇不良反应相关基因HLA-B*58∶01的检测
Rapid detection of HLA-B * 58∶01 gene based on multiplex melting curve analysis for the monitoring of adverse drug reaction caused by allopurinol
裴斌 1郭晓彤 2冉鹏 1黄秋英 2洪素云 1钟雪婷 2李庆阁2
作者信息
- 1. 厦门市中心血站,福建厦门361004
- 2. 厦门大学生命科学院
- 折叠
摘要
Abstract
Objective Evaluation of the HLA-B* 58∶01 gene detection based on multiplex melting curve analysis (MMCA) for the monitoring of adverse drug reaction caused by allopurinol.Methods We collected 1147 peripheral blood samples from donators in Xiamen City.DNA was extracted from the samples and a blind test was then performed to validate the consistency between human leukocyte antigens sequence-based typing (HLA-SBT) assay and MMCA assay for HLA-B* 58∶01 gene.Results In this study,213 cases were identified positive and 934 were negative by HLA-SBT assay and MMCA assay.The overall agreement between HLA-SBT assay and MMCA assay is 100％ (1147/1147) for HLA-B * 58∶01 gene detection.The percentage of positive samples was 18.57 ％ (213/1 147).Conclusion MMCA assay is a convenient and quick method with high sensitivity and excellent specificity and thus promises a powerful tool for the clinical diagnosis of HLA-B* 58∶01 gene.关键词
多色探针熔解曲线法/别嘌呤醇不良反应/HLA-B* 58∶01基因/痛风Key words
multiplex melting curve analysis (MMCA)/Adverse drug reaction caused by allopurinol/HLA-B * 58∶01 gene分类
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裴斌,郭晓彤,冉鹏,黄秋英,洪素云,钟雪婷,李庆阁..多色探针熔解曲线法应用于别嘌呤醇不良反应相关基因HLA-B*58∶01的检测[J].中国输血杂志,2018,31(1):37-40,4.
