中国动物传染病学报2018，Vol.26Issue(2)：9-14,6.
猪圆环病毒3型PCR检测方法的建立及其应用
DEVELOPMENT AND APPLICATION OF PCR METHOD FOR DETECTION OF PORCINE CIRCOVIRUS TYPE 3
摘要
Abstract
To detect porcine circovirus type 3(PCV3), an emerging porcine infectious agent, from clinical samples quickly and specifically, a pair of specific primers was designed and synthesized based on the complete genome sequence of PCV3 of GenBank to develop a PCR method. The amplified fragment was 932 bp in length. The classic plasmid concentration with PCR detection was 10 copy /μL for PCV3 but no amplification from nucleic acids of PCV1, PCV2, swine fever virus, Porcine reproductive and respiratory syndrome virus, Pseudorabies virus and Porcine parvovirus. The amplified fragments from 16 clinical samples of pigs were cloned and sequenced. The results showed that the nucleotide sequences shared 98.4%-99.6% and 99.0%-99.8% identity with the reported PCV3 SD2016 strain in the United States and Hubei-618 strain in China. The nucleotide homology between the obtained 16 strains was 98.3%-100%. Then, the PCR method was used to test 938 clinical samples collected from 2014 to 2017 in diseased pigs and 500 healthy pig samples in Jiangsu province. As a result, the positive rates of PCV3 were 6.93% and 0.6%, respectively. In addition, the positive rate was 1.92% in 2014 and then gradually increased to 12.67% in 2017. This study showed that the established PCR method is specific and sensitive and might be used for the detection of clinical PCV3 samples.关键词
圆环病毒3型/PCR/临床应用Key words
Porcine circovirus type 3/PCR/clinical development and application分类
农业科技引用本文复制引用
肖琦,李彬,朱雪蛟,胡屹屹,郭容利,曲梦,杨倩,何孔旺,茅爱华,汪伟,俞正玉,郭佳慧,袁万哲,赵攀登,温立斌,范宝超..猪圆环病毒3型PCR检测方法的建立及其应用[J].中国动物传染病学报,2018,26(2):9-14,6.基金项目
中国农业科学院基本科研业务费专项院级统筹工作项目(Y2017PT50) （Y2017PT50）
