中国动物传染病学报2018，Vol.26Issue(2)：22-27,6.
猴B病毒通用核酸检测体系的建立与评价
ESTABLISHMENT AND EVALUATION OF A UNIVERSAL NUCLEIC ACID TEST METHEOD FOR DETECTING MONKEY B VIRUS
摘要
Abstract
To establish and evaluate a universal real-time fluorescent quantitative PCR method for identification and quantification of 5 Monkey B virus (macacine alphaherpesvirus 1) genotypes, a set of suitable universal primers and probes were designed from the conserved region of the gB gene available in the NCBI nucleotide database.Then, plasmids were constructed and used to generate standard curves. The quantitative PCR method was developed and assessed for its specificity, sensitivity and reproducibility. A linear relationship was observed from 3×102copies to 3×107copies of Monkey B virus. The qPCR developed here did not amplify Herpes simplex virus 1, Herpes simplex virus 2, Epstein-barr virus, Varicella zoster virus and Cytomegalovirus. The detection limit of qPCR assay was 30 copies for 5 genotypes of Monkey B virus. Analyses of the inter-and intra-assay variabilities showed good reproducibility. The establishment of the universal real-time qPCR method for the detection of Monkey B virus DNA would facilitate diagnosis of Monkey B virus infection in humans and make great significance to the generation of high-quality experimental monkeys.关键词
猴B病毒/实时荧光定量PCR/核酸检测Key words
Monkey B virus/real-time fluorescent quantitative PCR/nucleic acid detection分类
农业科技引用本文复制引用
马丽敏,贾俊婷,钟亚迪,范瑞,马玉媛,刘兴友,章金刚..猴B病毒通用核酸检测体系的建立与评价[J].中国动物传染病学报,2018,26(2):22-27,6.基金项目
国家科技支撑计划项目(2015BAI07B00) （2015BAI07B00）
