中国动物传染病学报2018，Vol.26Issue(2)：34-39,6.
8株鸭疫里默氏杆菌安徽分离株的生物学特性分析
CHARACTERIZATION OF EIGHT ANHUI ISOLATES OF RIEMERELLA ANATIPESTIFER
摘要
Abstract
Riemerella anatipestifer(RA)is the causative agent of some epizootic diseases in poultry. In this study, the biological characteristics of 8 RA strains isolated from Anhui in 2017 were studied.The serotyping showed that 6 isolates belonged to serotype 10 and other two isolates belonged to serotype 15.The testing of drug susceptibilities using 24 antibiotics revealed that the RA isolates were sensitive to amoxicillin, ceftazidime and cefpyridine but resistant to lomefloxacin and azithromycin. Biofilm formation assay showed that 7 isolates produced strong biofilm while only one strain(AH-35)was weak in vitro.The mRNA levels of 5 genes associated with RA biofilm formation was analyzed between AH-1 (strong biofilm formation strain) and AH35 (weak biofilm formation strain) by real-time PCR.The results showed that Riean_0023,Riean_1039 and Riean_1564 were significantly increased(p<0.01)in the AH-1 compared with AH-35.This study will benefit to the prevention and control of RA in Anhui province.关键词
鸭疫里默氏杆菌/安徽分离株/药物敏感性/生物被膜/荧光定量PCRKey words
Riemerella anatipestifer/Anhui isolates/drug susceptibilities/biofilm/real-time PCR分类
农业科技引用本文复制引用
荆雅玮,陈芳芳,左佳坤,胡剑刚,石欣池,米荣升,黄燕,陈兆国,韩先干..8株鸭疫里默氏杆菌安徽分离株的生物学特性分析[J].中国动物传染病学报,2018,26(2):34-39,6.基金项目
上海市科技兴农重点攻关项目(沪农科攻字(2015)第1-9号) （沪农科攻字(2015）
