中国动物传染病学报2018，Vol.26Issue(2)：54-58,5.
永生化绵羊肺成纤维细胞系的构建及鉴定
ESTABLISHMENT AND IDENTIFICATION OF IMMORTALIZED SHEEP LUNG FIBROBLAST CELL LINE
摘要
Abstract
The lentivirus system carrying hTERT gene was inserted to sheep lung fibroblasts (SLT) in order to establish an immortal cell line. Firstly, the recombinant lentiviral expression vector (pLOV-puro-hTERT) carrying hTERT gene was transfected into 293T cells. Subsequently, the primary SLT cells were infected with the rescued lentiviruses. The positive SLT cells expressing hTERT were selected with puromycin and examined with RT-PCR and Western blot. The results showed that hTERT gene was stably expressed in SLT cells, indicated that an immortalized SLT cell line had been established.关键词
绵羊肺成纤维细胞/人端粒酶逆转录酶/永生化Key words
Sheep lung fibroblast cells/hTERT/immortalization分类
农业科技引用本文复制引用
刘腾,董丹丹,张莉,朱杰,缪秋红,刘光清..永生化绵羊肺成纤维细胞系的构建及鉴定[J].中国动物传染病学报,2018,26(2):54-58,5.基金项目
国家重点研发计划项目(2016YFD0500108) （2016YFD0500108）
国家自然科学基金项目(31502068) （31502068）
上海市科技兴农重点攻关项目(2016043) （2016043）
中央级公益性科研院所基本科研业务费专项(2016JB01、2017JB06) （2016JB01、2017JB06）
