中国动物传染病学报2018，Vol.26Issue(2)：59-63,5.
犬干扰素α2的原核表达及抗病毒活性分析
PROKARYOTIC EXPRESSION AND ANTIVIRAL ANALYSIS OF CANINE INTERFERON α 2
摘要
Abstract
In this study, the mature encoding sequence of canine interferon α 2 subtype was synthesized and inserted into the prokaryotic expression vector pET28a(+) to construct the recombinant plasmid pET-28a-CaIFN-α2. The resulting pET-28a-CaIFN-α2 was transformed into E.coli Rosetta competent cells for protein expression with induction of IPTG.The recombinant CaIFN-α2 was determined to be 23 kDa of molecular mass in SDS-PAGE and Western blot. In addition, the recombinant CaIFN-α2 mainly existed in a form of inclusion body and accounted for 52.5% of the total bacterial proteins. The recombinant CaIFN-α2 with the purity of 92% was obtained through a series of denaturation, renaturation and purification treatments. The antiviral activity of the recombinant CaIFN-α 2 was determined by CPE reduction of vesicular stomatitis virus infection in MDCK cells. The results showed that the antiviral activity of the recombinant CaIFN-α2 was 3.16×106U/mL, which laid the foundation for the development of novel canine interferon agent.关键词
重组犬干扰素α2/原核表达系统/抗病毒活性Key words
Recombinant canine interferon α2/prokaryotic expression system/antiviral activity分类
农业科技引用本文复制引用
姚凌云,芮荣,王永山,王晶宇,欧阳伟,钱晶,吴世妍,夏兴霞,诸玉梅,王晓丽,潘群兴..犬干扰素α2的原核表达及抗病毒活性分析[J].中国动物传染病学报,2018,26(2):59-63,5.基金项目
国家重点研发计划项目(2016YFD0501000) （2016YFD0501000）
国家公益性行业(农业)科研专项(201303042) （农业）
江苏省农业科技自主创新资金(CX(15)1065) （CX(15）
