中国动物传染病学报2018，Vol.26Issue(2)：80-84,5.
高效液相法检测鸡盲肠中的沙咪珠利
DETERMINATION OF ETHANAMIZURIL RESIDUE IN CHICKEN CAECUM USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
摘要
Abstract
The metabolism and elimination of a new anticoccidial drug ethanamizuril in chicken caecum was determined using high performance liquid chromatography. Separation was carried out on a X TERRA MS C18 column (Waters, 4.6×250 mm) with acetonitrile-0.2% phosphoric acid aqueous solution (v:v, 35:65) as a mobile phase. PDA UV detection was done at 251 nm. Toltrazuril sulfoxide was used as internal standard. Caecum samples of chickens were homogenized and ethanamizuril was extracted with acetonitrile followed by defatting with hexane. The detection level was linear over the range of 0.2~40 mg/kg and showed a good linear relationship (r2=0.994).The relative recovery was in the range of 90%~107%.The intra-and inter-day precisions(as coefficient of variation)were 2.37%~8.72% and 4.09%~6.80%. The results showed that the high performance liquid chromatography was a rapid, sensitive, and reliable method for detection of ethanamizuril in chicken caecum.关键词
抗球虫药/沙咪珠利/高效液相色谱/鸡盲肠Key words
Anticoccidial drugs/ethanamizuril/high performance liquid chromatography/chicken caecum分类
农业科技引用本文复制引用
易远胜,郭春娜,王霄旸,张可煜,杨帆帆,孙志良,薛飞群..高效液相法检测鸡盲肠中的沙咪珠利[J].中国动物传染病学报,2018,26(2):80-84,5.基金项目
国家科技支撑计划项目(2015BAD11B00) （2015BAD11B00）
国家自然科学基金项目(C180701) （C180701）
