中国实用口腔科杂志2018，Vol.11Issue(2)：79-83,5.DOI:10.19538/j.kq.2018.02.005
慢性牙周炎合并糖尿病患者唾液及龈下菌斑中牙周致病菌检测临床研究
Clinical study on detection of periodontal pathogens in saliva and gingival crevicular fluid of patients with chronic periodontitis complicated with diabetes mellitus
摘要
Abstract
Objective To investigate the differences in detection results of periodontal pathogens in saliva and gingival crevicular fluid between patients with chronic periodontitis (CP) and those complicated with diabetes mellitus (DM).Methods Of the patients treated in the First Affiliated Hospital of Henan University between Aug.2012 and Sep.2015,40 cases of patients with CP complicated with T2DM were selected as CP+T2DM group,65 cases of patients with simple CP were selected as CP group,and 35 cases of healthy subjects were selected as the control group.The kinds and relative contents of suspected pathogens,such as Porphyromonas gingiwdis (Pg),Actinobacitlus actinomycetemcomitans (Aa),Fusobacterium nucleatum (Fn),Tannerella forsythia (Tf),intermediate type Prevotella intermedia (Pi) and Treponema denticola(Td),in saliva and gingival crevicular fluid were detected by polymerase chain reaction(PCR).Results The detection rates of Pg,Aa and Tf in the saliva and gingival crevicular fluid showed statistically significant differences among the three groups (P ＜ 0.05),but there were no significant differences in the detection rates of Fn,Pi or Td (P ＞ 0.05).The detection rate of Fn in saliva and gingival crevicular fluid of the three groups was the highest,and the detection rates of Pg,Aa and Tfin saliva and gingival crevicular fluid of CP +T2DM group were significantly higher than those in control group and CP group (P ＜ 0.05).The detection rate of Pg in CP group was significantly higher than that in the control group (P ＜ 0.05).The relative contents of Pg,Aa and Tf in the saliva and gingival crevicular fluid of CP+T2DM group and CP group were significantly higher than those in the control group (P ＜ 0.05).The relative contents of Aa and Tf in saliva and gingival crevicular fluid of CP + T2DM group were significantly higher than those in CP group (P ＜ 0.05).Conclusion There are significant differences in the types and number of suspected pathogens between patients with simple CP and those with CP and DM.Pg,Aa and Tf have more advantages in patients with DM,which may be closely related to DM.关键词
慢性牙周炎/糖尿病/唾液/龈下菌斑/牙周致病菌Key words
chronic periodontitis/diabetes mellitus/saliva/gingival crevicular fluid/periodontal pathogens分类
医药卫生引用本文复制引用
苏娟娟,薛鹏..慢性牙周炎合并糖尿病患者唾液及龈下菌斑中牙周致病菌检测临床研究[J].中国实用口腔科杂志,2018,11(2):79-83,5.基金项目
国家自然科学基金项目(81100759) （81100759）
