中国实用口腔科杂志2018，Vol.11Issue(2)：91-94,4.DOI:10.19538/j.kq.2018.02.008
声动力疗法辅助治疗大鼠牙周炎效果研究
Sonodynamic therapy as an adjuvant treatment for periodontitis in rats
摘要
Abstract
Objective To evaluate the effect of HMME-mediated adjunctive sonodynamic therapy (SDT)on periodontitis in rats,in order to provide theoretical and experimental evidence for the clinical application of SDT.Methods Ligatures were placed on the subgingival regions of the first maxillary molar in 54 rats to induce the periodontitis models.Then the rats were randomly divided into three groups with 18 rats in each group.(1)The control group was treated by normal saline (NS) to wash the periodontal pocket;(2)the scaling and root planing (SRP)group received SRP and was washed with normal saline;(3)the SRP plus SDT group was treated by SRP and washed with normal saline,and then was treated through subcutaneous injection of HMME 1 mL(40 μg/mL).After 90 min of incubation in the dark,the ultrasonic treatment at the frequency of 1 MHz and the intensity of 3W/cm2 was given for 10 minutes.SDT was performed every other day.Six rats from each group were sacrificed at 6,10,or 14 days postoperatively and periodontal tissue samples were taken for histological examination and immunohistochemical analysis.Results SRP plus SDT group had reduced number of inflammatory cells.The expression of receptor activator of nuclear factor kappa-B ligand (RANKL)was weakly positive,and the number of positive Tartrate-resistant acid phosphatase (TRAP) was significantly reduced (P ＜0.05),compared to the SRP and the control group (P ＜ 0.05).Conclusion SDT is an effective adjunctive therapy for periodontitis in rats.关键词
牙周炎/声动力疗法/血卟啉单甲醚/洁治和刮治Key words
periodontitis/sonodynamic therapy/hematoporphyrin monomethyl ether/scaling分类
医药卫生引用本文复制引用
栾小敏,庄德舒,代佳音,路海艳,毕良佳..声动力疗法辅助治疗大鼠牙周炎效果研究[J].中国实用口腔科杂志,2018,11(2):91-94,4.基金项目
国家自然科学基金(81670994) （81670994）
