中国现代医学杂志2018，Vol.28Issue(11)：5-9,5.DOI:10.3969/j.issn.1005-8982.2018.011.002
胃浆膜下层与门静脉胰岛移植治疗糖尿病大鼠的实验研究
Experimental study on gastric subserosa and portal vein islet transplantation for treatment of rat diabetes
刘国涛 1郭栋 1刘全达 2程宇 1周丁华2
作者信息
- 1. 锦州医科大学火箭军总医院研究生培养基地,辽宁 锦州 121001
- 2. 中国人民解放军火箭军总医院,北京 100088
- 折叠
摘要
Abstract
Objective To explore the most suitable transplant site for islet transplantation by comparing the efficacy of gastric subserosal transplantation and portal vein islet transplantation in the treatment of diabetic rats. Methods Streptozotocin-induced diabetic rats were divided into gastric subserous group (n = 6), portal vein group (n = 6) and control group (n = 6). Type XI collagenase was injected through choledoch to digest the pancreas, and the islets were isolated and purified. The specificity of the islet was judged by dithizone (DTZ) staining, and IEQ and islets purity were counted. The activity of islets was determined by trypan blue test. 500IEQ fresh islets were transplanted into the gastric subserosa and into the portal vein, respectively. The control group was infused with the same amount of RPMI 1640. After islet grafting in the diabetic rats, the changes in blood glucose and intraperitoneal glucose tolerance were evaluated to assess the function of islets. Results The number of islets was (310 ± 42) in each transplanted rat, and the purity of islets was (89 ± 4.52)% obtained by DTZ staining and the activity of islets was (89.08 ± 3.76)% obtained by trypan blue staining. The glucose levels in the gastric subserous and portal vein groups were significantly decreased after transplantation compared with those before transplantation (P < 0.05) and significantly different from that in the control group (P < 0.05). After transplantation the average blood glucose level of the gastric subserous group was significantly lower than that of the portal vein group (P < 0.05). The time of effective control of blood glucose in the gastric subserous group and the portal vein group was (18.0 ± 1.9) d and (11.6 ± 2.5) d (P < 0.05). The rats of the gastric subserous and portal vein groups had good intraperitoneal glucose tolerance. Conclusions In the short term, the effect of gastric subserous islet transplantation for treatment of rat diabetes is better than that of portal vein islet transplantation. Compared with portal vein islet transplantation, the operation of gastric subserous pancreatic islet transplantation is simple and safe, and gastric subserosa can be considered as one of the most suitable parts of islet transplantation.关键词
糖尿病大鼠/胰岛移植/胃浆膜下层/门静脉Key words
diabetic rat/islet transplantation/gastric subserosa/portal vein分类
医药卫生引用本文复制引用
刘国涛,郭栋,刘全达,程宇,周丁华..胃浆膜下层与门静脉胰岛移植治疗糖尿病大鼠的实验研究[J].中国现代医学杂志,2018,28(11):5-9,5.
