中国现代医学杂志2018，Vol.28Issue(11)：10-14,5.DOI:10.3969/j.issn.1005-8982.2018.011.003
三七总皂苷对过氧化氢诱导HT22细胞氧化损伤的保护作用
Protective effect of panax notoginseng saponins on oxidative injury induced by hydrogen peroxide in HT22 cells
摘要
Abstract
Objective To study the protective effect of panax notoginseng saponins (PNS) on hippocampal neurons HT22 cells after injury by hydrogen peroxide (H2O2). Methods CCK-8 was used to detect the cell growth activity of HT22 cells after the action of PNS. After stimulation with H2O2, the effect of PNS on the activity and morphological changes of HT22 cells were observed. Cell damage was checked using LDH test. Flow cytometry was used to count the percentage of apoptotic cells. Results Panax notoginseng saponins had no toxic effect on HT22 cells in effective concentration range, and could enhance the cell vitality, significantly reduce the cell damage caused by H2O2. Conclusions Panax notoginseng saponins can enhance the intracellular antioxidant activity to enhance the survival rate of HT22 cells, thus protect the cells from oxidative damage.关键词
三七总皂苷/HT22细胞/氧化损伤Key words
panax notoginseng saponins/HT22 cell/oxidative damage分类
医药卫生引用本文复制引用
韩峰,颜楠,李侠,费舟..三七总皂苷对过氧化氢诱导HT22细胞氧化损伤的保护作用[J].中国现代医学杂志,2018,28(11):10-14,5.基金项目
国家自然科学基金重点资助项目(No:30930093) （No:30930093）
