中国现代医学杂志2018，Vol.28Issue(11)：15-21,7.DOI:10.3969/j.issn.1005-8982.2018.011.004
IGHG1基因对膀胱癌细胞增殖凋亡及迁移的影响
Effect of IGHG1 gene on cell migration,proliferation and apoptosis of bladder cancer cells
摘要
Abstract
Objective To investigate the effect of IGHG1 gene silencing on migration, proliferation and apoptosis of human bladder cancer EJ cells. Methods Bladder cancer cell line EJ cells were transfected with LV-IGHG1-RNAi and taken as experimental group(shIGHG1-EJ cells).Negative scrambled lentiviral vector was used to transfect EJ cells, which were treated as negative control group (NC-EJ cells). EJ cells without transfection were used to be blank control group (EJ cells). The expressions of IgG γ, caspase-3 and cleaved caspase-3 were detected by Western blot and IGHG1 mRNA expression by qRT-PCR.CCK-8 was used to detect the proliferation of the three groups of cells. Flow cytometry (FCM) was used to detect the cell apoptosis. Wound healing assay was used to investigate the change in migration of shIGHG1-EJ cells. Results The transfection rate of the shIGHG1-EJ cells was (76.667 ± 0.042)%, and the transfection rate of the NC-EJ cells was (75.333 ± 0.055)%. The expressions of IGHG1 mRNA and IgG γ of the shIGHG1-EJ group were obviously lower than those of the control groups(P<0.05),which showed that IGHG1 gene silencing was successful.CCK-8 revealed that IGHG1 gene silencing significantly decreased the proliferation of EJ cells(P<0.05).FCM and Western blot demonstrated that IGHG1 gene silencing promoted the apoptosis of EJ cells (P < 0.05), and the cleaved caspase-3 fragment appeared at 17 kda. Wound healing assay showed that cell migration was inhibited by IGHG1 gene silencing (P< 0.05). However, NC-EJ cells had no meaningful difference from EJ cells(P>0.05). Conclusions IGHG1 gene silencing can observably inhibit the migration and proliferation of human bladder cancer EJ cells. It can also promote cell apoptosis.关键词
膀胱肿瘤/基因沉默/IGHG1/增殖/细胞凋亡Key words
bladder neoplasm/gene silencing/IGHG1/proliferation/apoptosis分类
医药卫生引用本文复制引用
吴遥西,李浩勇,周治彦,陈金火,李琦,马哲,金应霞,梁培育..IGHG1基因对膀胱癌细胞增殖凋亡及迁移的影响[J].中国现代医学杂志,2018,28(11):15-21,7.基金项目
国家自然科学基金(No:81360375) （No:81360375）
海南省社会发展重大专项课题(No:2015SF31) （No:2015SF31）
