中国现代医学杂志2018，Vol.28Issue(11)：22-26,5.DOI:10.3969/j.issn.1005-8982.2018.011.005
凤冈富锌硒茶对ERp57蛋白酶的抑制作用及其有效成分的虚拟筛选
Inhibitory effect of Se-enriched and zinc-enriched green tea of Fenggang on ERp57 protease and virtual screening of its effective constituents
摘要
Abstract
Objective To examine the effect of Se-enriched and zinc-enriched green tea of Fenggang on the enzymatic activity of ERp57, a potential anti-platelet target, and try to clarify the major constituents of the extract. Methods Ultra performance liquid chromatography (UPLC) was used to assay the major components of the green tea. The effect of the extract on platelet function and ERp57 activity was evaluated by light transmission aggregometry and insulin reduction assay, respectively. The major active constituents of the green tea were identified using virtual screening and experimental validation. Results Using UPLC, 4 major components were identified from the tea extract. Further experiments with insulin turbidity assay and virtual screening using computer-based methods demonstrated the green tea extract displayed potent inhibitory effect on the enzymatic activity of ERp57 (among which EGCG had the strongest effect) and certain anti-platelet aggregation effect. Conclusions The beneficial effect of green tea on the mortality of CVD patients could be associated with its inhibition of ERp57 activity and platelet aggregation. In addition, EGCG is one of the major active constituents of the green tea.关键词
凤冈富锌硒茶/ERp57/心血管疾病/虚拟筛选/抗血小板聚集Key words
Se-enriched and zinc-enriched green tea of Fenggang/ERp57/cardiovascular disease/virtual screening/anti-platelet aggregation分类
医药卫生引用本文复制引用
张莹,李惠琼,邹佳,王滔,李玲玲,申慧芳,岳续朋,崔国祯..凤冈富锌硒茶对ERp57蛋白酶的抑制作用及其有效成分的虚拟筛选[J].中国现代医学杂志,2018,28(11):22-26,5.基金项目
国家自然科学基金(No:81460552) （No:81460552）
遵义医学院招标课题(No:F-787) （No:F-787）
贵州省教育厅优秀科技创新人才奖励计划[No:黔教合KY字(2015)494] （2015）
